Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey

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dc.contributor.authorPetras, Daniel
dc.contributor.authorHempel, Benjamin-Florian
dc.contributor.authorGocmen, Bayram
dc.contributor.authorKaris, Mert
dc.contributor.authorWhiteley, Gareth
dc.contributor.authorWagstaff, Simon C.
dc.contributor.authorHeiss, Paul
dc.contributor.authorCasewell, Nicholas R.
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorSuessmuth, Roderich D.
dc.date.accessioned2019-10-27T09:44:20Z
dc.date.available2019-10-27T09:44:20Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractWe report on the variable venom composition of a population of the Caucasus viper (Vipera kaznakovi) in Northeastern Turkey. We applied a combination of venom gland transcriptomics, de-complexing bottom-up and top-down venomics. In contrast to sole bottom-up venomics approaches and gel or chromatography based venom comparison, our combined approach enables a faster and more detailed comparison of venom proteomes from multiple individuals. In total, we identified peptides and proteins from 15 toxin families, including snake venom metalloproteinases (svMP; 37.8%), phospholipases A(2) (PLA(2); 19.0%), snake venom serine proteinases (svSP; 11.5%), C-type lectins (CTL; 6.9%) and cysteine-rich secretory proteins (CRISP; 5.0%), in addition to several low abundant toxin families. Furthermore, we identified intraspecies variations of the venom composition of V. kaznakovi, and find these were mainly driven by the age of the animals, with lower svSP abundance detected in juveniles. On the proteoform level, several small molecular weight toxins between 5 and 8 kDa in size, as well as PLA(2)s, drove the differences observed between juvenile and adult individuals. This study provides novel insights into the venom variability of V. kaznakovi and highlights the utility of intact mass profiling for fast and detailed comparison of snake venom. Biological significance: Population level and ontogenetic venom variation (e.g. diet, habitat, sex or age) can result in a loss of antivenom efficacy against snakebites from wide ranging snake populations. The current state of the art for the analysis of snake venoms are de-complexing bottom-up proteomics approaches. While useful, these have the significant drawback of being time-consuming and following costly protocols, and consequently are often applied to pooled venom samples. To overcome these shortcomings and to enable rapid and detailed profiling of large numbers of individual venom samples, we integrated an intact protein analysis workflow into a transcriptomics-guided bottom-up approach. The application of this workflow to snake individuals of a local population of V. kaznakovi revealed intraspecies variations in venom composition, which are primarily explained by the age of the animals, and highlighted svSP abundance to be one of the molecular drivers for the compositional differences observed.en_US
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG) through the Cluster of Excellence 'Unifying Concepts in Catalysis (UniCat)German Research Foundation (DFG); Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Z946]; Sir Henry Dale Fellowship - Wellcome Trust [200517/Z/16/Z]; Sir Henry Dale Fellowship - Royal Society [200517/Z/16/Z]; [PE 2600/1]en_US
dc.description.sponsorshipWe would like to thank Ricardo Da Silva for help with the multi variable statistics, Sabah Ul-Hasan and Anthony Saviola for critical reading of the paper draft, and Robert Harrison for assistance with transcriptomics. This study was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Cluster of Excellence 'Unifying Concepts in Catalysis (UniCat) and a postdoctoral research scholarship to D.P. (PE 2600/1), the Scientific and Technical Research Council of Turkey (TUBITAK) under Grant 114Z946, and a Sir Henry Dale Fellowship (200517/Z/16/Z) jointly funded by the Wellcome Trust and the Royal Society to N.R.C.en_US
dc.identifier.doi10.1016/j.jprot.2019.02.004en_US
dc.identifier.endpage50en_US
dc.identifier.issn1874-3919
dc.identifier.issn1876-7737
dc.identifier.pmid30763806en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://doi.org/10.1016/j.jprot.2019.02.004
dc.identifier.urihttps://hdl.handle.net/11454/28986
dc.identifier.volume199en_US
dc.identifier.wosWOS:000464296800003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Proteomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectViperidaeen_US
dc.subjectSnakeen_US
dc.subjectMiddle Easten_US
dc.subjectToxinen_US
dc.subjectCaucasus viperen_US
dc.subjectVipers kaznakovien_US
dc.subjectSnake Venomicsen_US
dc.subjectTranscriptomicsen_US
dc.subjectTop-down Venomicsen_US
dc.subjectPopulation Venomicsen_US
dc.subjectVenomen_US
dc.subjectToxinen_US
dc.titleIntact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkeyen_US
dc.typeArticleen_US

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