Results of a multicenter study investigating plasmid mediated colistin resistance genes (mcr-1 and mcr-2) in clinical enterobacteriaceae isolates from Turkey [Ülkemizde Klinik Enterobacteriaceae Izolatlarinda Plazmit Aracili Kolistin Direnç Genlerini (mcr-1 ve mcr-2) Araştiran Çok Merkezli Çalişmaya Ait Sonuçlar]

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dc.contributor.authorSari A.N.
dc.contributor.authorSüzük S.
dc.contributor.authorKararina O.
dc.contributor.authorÖgünç D.
dc.contributor.authorKarakoç A.E.
dc.contributor.authorÇizmeci Z.
dc.contributor.authorAlişkan H.E.
dc.contributor.authorCömert F.
dc.contributor.authorBakici M.Z.
dc.contributor.authorAkpolat N.
dc.contributor.authorÇilli F.F.
dc.contributor.authorZer Y.
dc.contributor.authorKarataş A.
dc.contributor.authorKarapinar B.A.
dc.contributor.authorBayramoglu G.
dc.contributor.authorÖzdamar M.
dc.contributor.authorKalem F.
dc.contributor.authorDelialioglu N.
dc.contributor.authorAktaş E.
dc.contributor.authorYilmaz N.
dc.contributor.authorGürcan S.
dc.contributor.authorGülay Z.
dc.date.accessioned2019-10-27T08:03:50Z
dc.date.available2019-10-27T08:03:50Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractColistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmidmediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmidmediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae isolates from different parts of our country. A total of 329 Enterobacteriaceae isolates from 22 laboratories were collected which were isolated between March, 2015 and February, 2016. mcr-1/mcr-2 were investigated by polymerase chain reaction during February-March, 2016. Two hundred and seventeen of Klebsiella pneumoniae (66%), 75 of Salmonella spp. (22.8%), 31 of Esherichia coli (9.4%), 3 of Enterobacter cloacae (0.9%), 2 of Klebsiella oxytoca (0.6%) and 1 of Enterobacter aerogenes (0.3%) isolates were included to the study. Agarose gel electrophoresis results of PCR studies have shown expected band sizes for positive control isolates as 309 bp for mcr-1 and 567 bp for mcr-2. However, the presence of mcr-1/mcr-2 genes was not detected among the tested study isolates of Enterobacteriaceae. Although mcr-1/mcr-2 were not detected in our study isolates, it is highly important to understand the mechanism of resistance dissemination and determine the resistant isolates by considering that colistin is a last-line antibiotic against infections of multidrug or carbapenem resistant gram-negative bacteria. Thus, it is suggested that these mechanisms should be followed-up in both clinical and non-clinical (e.g. isolates from food animals, raw meats and environment) isolates of special populations.en_US
dc.identifier.doi10.5578/mb.57515en_US
dc.identifier.endpage303en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid28929967en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage299en_US
dc.identifier.urihttps://doi.org/10.5578/mb.57515
dc.identifier.urihttps://hdl.handle.net/11454/25424
dc.identifier.volume51en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Societyen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectColistinen_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectMcr-1en_US
dc.subjectMcr-2en_US
dc.titleResults of a multicenter study investigating plasmid mediated colistin resistance genes (mcr-1 and mcr-2) in clinical enterobacteriaceae isolates from Turkey [Ülkemizde Klinik Enterobacteriaceae Izolatlarinda Plazmit Aracili Kolistin Direnç Genlerini (mcr-1 ve mcr-2) Araştiran Çok Merkezli Çalişmaya Ait Sonuçlar]en_US
dc.typeArticleen_US

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