Polymer Coated Iron Nanoparticles: Radiolabeling & In vitro Studies

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dc.authoridIchedef, Cigdem/0000-0002-1586-9521
dc.authoridYilmaz, Selin/0000-0001-7958-9927
dc.authoridKARATAY, Kadriye Busra/0000-0002-2811-4689
dc.contributor.authorYilmaz, Selin
dc.contributor.authorIchedef, Cigdem
dc.contributor.authorKaratay, Kadriye Busra
dc.contributor.authorTeksoz, Serap
dc.date.accessioned2023-01-12T20:11:44Z
dc.date.available2023-01-12T20:11:44Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractBackground: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it has been aimed to develop a novel targeted Tc-99m radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with Tc-99m. SPIONs were synthesized by reduction-coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM), respectively. The radiolabeling yield of SPION-PLGAGEM nanoparticles was determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles was deter- mined as 366.6 nm by DLS, while zeta potential was found as 29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4 +/- 0.16% by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles was determined as 97.8 +/- 1.75% via TLRC. Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, while the incorporation rate was increased for both cell lines with external magnetic field application. Conclusion: Tc-99m labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and super-paramagnetic characteristicsen_US
dc.description.sponsorshipCelal Bayar University Scientific Research Projects Coordination Unit [2017-001]en_US
dc.description.sponsorshipCelal Bayar University Scientific Research Projects Coordination Unit (Grant Number: 2017-001).en_US
dc.identifier.doi10.2174/1874471013666200430094113
dc.identifier.endpage45en_US
dc.identifier.issn1874-4710
dc.identifier.issn1874-4729
dc.identifier.issue1en_US
dc.identifier.pmid32351192en_US
dc.identifier.startpage37en_US
dc.identifier.urihttps://doi.org/10.2174/1874471013666200430094113
dc.identifier.urihttps://hdl.handle.net/11454/78181
dc.identifier.volume14en_US
dc.identifier.wosWOS:000641557600006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofCurrent Radiopharmaceuticalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetic drug deliveryen_US
dc.subjectpolymeric nanoparticlesen_US
dc.subjectgemcitabineen_US
dc.subjectradiolabelingen_US
dc.subjectTc-99men_US
dc.subjectin vitroen_US
dc.titlePolymer Coated Iron Nanoparticles: Radiolabeling & In vitro Studiesen_US
dc.typeArticleen_US

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