Purification, characterization and in vivo biocontrol efficiency of killer toxins from Debaryomyces hansenii strains

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dc.contributor.authorCorbaci, Cengiz
dc.contributor.authorUcar, Fusun B.
dc.date.accessioned2019-10-27T10:01:15Z
dc.date.available2019-10-27T10:01:15Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractNowadays, the biological control of various yeast and mold pathogens that cause diseases in humans, animals, and plants is an increasing of interest. The discovery of novel agents allows prevention of infectious diseases and post-harvest losses reported every year. In the study, we aimed to investigate the production, purification, and characterization as well as in vivo biocontrol efficiency of killer toxins produced by Debaryomyces hansenii strains TEM8 and TEM17. The molecular mass of the killer toxins was 31.5 kDa and they showed high stability at pHs between 2.5 and 5.5 and up to 37 degrees C. Their internal amino acid sequences matched the DEHA2G18766g (CAG90862.1) from D. hansenii CBS767, which is similar to Saccharomyces cerevisiae YGR282C BGL2 endo-beta-1,3-glucanase. The yeasts and their purified killer toxins significantly inhibited the growth of plant pathogenic fungi Alternaria brassicicola, Alternaria citri, Aspergillus niger and Rhizopus stolonifer in fruits. The findings of this paper have recommended these yeast strains and their toxins as effective biocontrol agents against fungi that cause post-harvest diseases. (C) 2018 Published by Elsevier B.V.en_US
dc.description.sponsorshipScientific Research Unit of Ege UniversityEge University [11-FEN-076]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Unit of Ege University (11-FEN-076). We would like to extend our thanks to the Proteome Factory AG for performing protein identification analysis.en_US
dc.identifier.doi10.1016/j.ijbiomac.2018.07.121en_US
dc.identifier.endpage1082en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid30031822en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1077en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2018.07.121
dc.identifier.urihttps://hdl.handle.net/11454/29817
dc.identifier.volume119en_US
dc.identifier.wosWOS:000447682100120en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectD. hanseniien_US
dc.subjectKiller toxinen_US
dc.subjectBiocontrolen_US
dc.titlePurification, characterization and in vivo biocontrol efficiency of killer toxins from Debaryomyces hansenii strainsen_US
dc.typeArticleen_US

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