Detection of rifampin resistance in Mycobacterium tuberculosis strains by RNA/RNA mismatch method [Mycobacterium tuberculosis i·zolatlarinda ri·fampi·n di·renci·ni·n RNA/RNA uyumsuz bi·rleşme (mismatch) yöntemi· i·le saptanmasi]
Küçük Resim Yok
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Rifampin is one of the most potent antituberculosis drugs and therefore, rifampin resistance leads to high clinical relapse rates. Detection of rifampin resistance could be an indication of multidrug resistance. In the recent years several molecular methods have been developed to evaluate the mutations in rpoB gene for the detection of rifampin resistance. The aim of the present study was to evaluate the performance of the RNA/RNA Mismatch Assay for detection of the mutations in the rpoB gene in 20 M.tuberculosis isolates which were determined as resistant to rifampin by agar proportion method. While RNA/RNA Mismatch Assay detected the mutations in the rpoB region in 16 of 20 (80%) M.tuberculosis isolates, the remaining four isolates yielded no band pattern indicating resistance. However, there may be situations where interpretation of the results is difficult in RNA/RNA Mismatch Assay which is already cheaper than DNA sequencing and other molecular methods. In conclusion, if the RNA/RNA Mismatch Assay can be optimized, it can be used for the rapid detection of rifampin resistance.
Açıklama
Anahtar Kelimeler
Mycobacterium tuberculosis, Rifampin resistance, RNA/RNA Mismatch Assay, rpoB
Kaynak
Mikrobiyoloji Bulteni
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
37
Sayı
1
