Affinity-Based Magnetic Nanoparticle Development for Cancer Stem Cell Isolation

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dc.authoridUlucan Karnak, Dr. Fulden/0000-0001-5567-0261
dc.authoridAkgöl, Sinan/0000-0002-8528-1854
dc.authoridSendemir, Aylin/0000-0003-1818-6651
dc.authoridKuru, Cansu Ilke/0000-0003-4290-6823
dc.contributor.authorKuru, Cansu Ilke
dc.contributor.authorUlucan-Karnak, Fulden
dc.contributor.authorDayioglu, Buesra
dc.contributor.authorSahinler, Mert
dc.contributor.authorSendemir, Aylin
dc.contributor.authorAkgol, Sinan
dc.date.accessioned2024-08-31T07:49:38Z
dc.date.available2024-08-31T07:49:38Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractCancer is still the leading cause of death in the world despite the developing research and treatment opportunities. Failure of these treatments is generally associated with cancer stem cells (CSCs), which cause metastasis and are defined by their resistance to radio- and chemotherapy. Although known stem cell isolation methods are not sufficient for CSC isolation, they also bring a burden in terms of cost. The aim of this study is to develop a high-efficiency, low-cost, specific method for cancer stem cell isolation with magnetic functional nanoparticles. This study, unlike the stem cell isolation techniques (MACS, FACS) used today, was aimed to isolate cancer stem cells (separation of CD133+ cells) with nanoparticles with specific affinity and modification properties. For this purpose, affinity-based magnetic nanoparticles were synthesized and characterized by providing surface activity and chemical reactivity, as well as making surface modifications necessary for both lectin affinity and metal affinity interactions. In the other part of the study, synthesized and characterized functional polymeric magnetic nanoparticles were used for the isolation of CSC from the human osteosarcoma cancer cell line (SAOS-2) with a cancer stem cell subpopulation bearing the CD133 surface marker. The success and efficiency of separation after stem cell isolation were evaluated via the MACS and FACS methods. As a result, when the His-graft-mg-p(HEMA) nanoparticle was used at a concentration of 0.1 mu g/mL for 106 and 108 cells, superior separation efficiency to commercial microbeads was obtained.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, 1005-National New Ideas and New Products Research Funding Program [116Z207]en_US
dc.description.sponsorshipThis study was funded by Scientific and Technological Research Council of Turkey, 1005-National New Ideas and New Products Research Funding Program, Project Number: 116Z207. The authors would like to thank to Scientific and Technological Research Council of Turkey for their financial support.en_US
dc.identifier.doi10.3390/polym16020196
dc.identifier.issn2073-4360
dc.identifier.issue2en_US
dc.identifier.pmid38256995en_US
dc.identifier.scopus2-s2.0-85183079166en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/polym16020196
dc.identifier.urihttps://hdl.handle.net/11454/104932
dc.identifier.volume16en_US
dc.identifier.wosWOS:001151057900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPolymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectMagnetic Nanoparticleen_US
dc.subjectCancer Stem Cellen_US
dc.subjectAffinity Interactionsen_US
dc.subjectStem Cell Isolationen_US
dc.titleAffinity-Based Magnetic Nanoparticle Development for Cancer Stem Cell Isolationen_US
dc.typeArticleen_US

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