Molecular screening of Leishmania spp. infection and bloodmeals in sandflies from a leishmaniasis focus in southwestern Turkey

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dc.contributor.authorKarakus, M.
dc.contributor.authorPekagirbas, M.
dc.contributor.authorDemir, S.
dc.contributor.authorEren, H.
dc.contributor.authorToz, S.
dc.contributor.authorOzbel, Y.
dc.date.accessioned2019-10-27T11:07:59Z
dc.date.available2019-10-27T11:07:59Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractLeishmaniasis is an arthropod-borne disease that affects approximately 2 million people worldwide annually. The aims of this study were to detect the presence of Leishmania (Kinetoplastida: Trypanosomatidae) DNA and the feeding preferences of probable vector species in an endemic focus of Leishmania infantum in Turkey. Entomological sampling was performed in August and October 2015 in Aydn province, where cases of human and canine leishmaniasis have been reported previously. A total of 1059 sandfly specimens comprising nine species belonging to two genera, Phlebotomus and Sergentomyia (both: Diptera: Psychodidae), and five subgenera of the Phlebotomus genus (Phlebotomus, Paraphlebotomus, Larroussius, Adlerius and Transphlebotomus) were collected in five villages. Among all Phlebotomus specimens, Phlebotomus neglectus (39%) was noted as the most abundant species, followed by Phlebotomus tobbi (18%). LeishmaniaDNA was detected in pools from P.neglectus, P.tobbi and Sergentomyia dentata by kDNA polymerase chain reaction (PCR). LeishmaniaDNA from Phlebotomus specimens was identified as L.infantum, but LeishmaniaDNA from Sergentomyia spp. could not be identified to species level by ITS-1 real-time PCR. The detection of LeishmaniaDNA in wild-caught P.neglectus and the high percentage (24.2%) of human DNA in engorged specimens suggests that P.neglectus is probably an important vector species for L.infantum in Aydn province.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114S999]en_US
dc.description.sponsorshipThe authors wish to dedicate this study to Professor Dr Mehmet Ali Ozcel, honorary president and founder of the Department of Parasitology at Ege University Medical School. The authors would like to thank Bahtiyar Yilmaz (Genmar Company, Izmir, Turkey) for his assistance in the bloodmeal study and Gozde Sir (Ege University, Medical School, Izmir, Turkey) for her technical support. This study was partially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (project no. 114S999).en_US
dc.identifier.doi10.1111/mve.12216en_US
dc.identifier.endpage229en_US
dc.identifier.issn0269-283X
dc.identifier.issn1365-2915
dc.identifier.issue2en_US
dc.identifier.pmid27910105en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage224en_US
dc.identifier.urihttps://doi.org/10.1111/mve.12216
dc.identifier.urihttps://hdl.handle.net/11454/32108
dc.identifier.volume31en_US
dc.identifier.wosWOS:000400031700013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofMedical and Veterinary Entomologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeishmaniaen_US
dc.subjectSauroleishmaniaen_US
dc.subjectSergentomyiaen_US
dc.subjectbloodmeal analysisen_US
dc.subjectTurkeyen_US
dc.titleMolecular screening of Leishmania spp. infection and bloodmeals in sandflies from a leishmaniasis focus in southwestern Turkeyen_US
dc.typeArticleen_US

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