Chemotherapy influences inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) activity on 3D breast cancer cell line

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dc.contributor.authorOktem, G.
dc.contributor.authorBilir, A.
dc.contributor.authorSelvi, N.
dc.contributor.authorYurtseven, M. E.
dc.contributor.authorVatansever, S.
dc.contributor.authorAtes, U.
dc.contributor.authorUysal, A.
dc.contributor.authorOmay, S. B.
dc.date.accessioned2019-10-27T19:21:01Z
dc.date.available2019-10-27T19:21:01Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractMulticellular tumor spheroids (NITS) are three-dimensional structural forms of tumors grown in vitro in the laboratory. In this study, the aim was to determine the regulation of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) expressions on NITS in response to treatment with the commonly used anti-cancer drugs Doxorubicin and Docetaxel. The spheroids were generated using the "liquid overlay" technique. The distribution of both iNOS and eNOS was detected using indirect immunohistochemistry, while the expression of both iNOS and eNOS was measured using Western blots. Additionally, S-phase analysis using 5-bromo-2'-deoxyuridine (BrdU) was done on the MTS after treatment with doxorubicin, docetaxel, and a combination of the two. The Griess method was used to measure nitric oxide (NO) production in the cells. An increase in iNOS immunoreactivity and a decrease in eNOS immunoreactivity were observed after doxorubicin treatment, when compared with the other groups. Furthermore, upregulation of iNOS and downregulation of eNOS were detected in doxorubicin-treated cells using Western blotting. Insignificant iNOS expression was observed in all of the groups, and it was particularly low in the control and drug combination groups. NO production was also found to be significantly high after docetaxel treatment, and cell proliferation decreased after doxorubicin treatment. In conclusion, chemotherapy influences NOS activity differently with the presence of different drugs. The results with iNOS show that doxorubicin is a more effective drug than docetaxel, and a drug combination may play a helpful role in the suppression of tumorigenicity and cancer metastasis. Interestingly, eNOS expression increased after the addition of both docetaxel and the drug combination, and it was found to negatively correlate with the histological grade of the tumor. Therefore, analyzing the expression of both iNOS and eNOS might be very useful for targeting the treatment of breast carcinoma and obtaining better information on prognosis.en_US
dc.identifier.endpage203en_US
dc.identifier.issn0965-0407
dc.identifier.issue4en_US
dc.identifier.pmid17120617en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage195en_US
dc.identifier.urihttps://hdl.handle.net/11454/38942
dc.identifier.volume16en_US
dc.identifier.wosWOS:000241459900003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCognizant Communication Corpen_US
dc.relation.ispartofOncology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectiNOSen_US
dc.subjecteNOSen_US
dc.subjectbreast canceren_US
dc.subjectchemotherapyen_US
dc.titleChemotherapy influences inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) activity on 3D breast cancer cell lineen_US
dc.typeArticleen_US

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