INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS; [PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EKSTRELERİNİN HEPG2 HÜCRELERİNDEKİ SİTOTOKSİK VE APOPTOTİK ETKİLERİNİN ARAŞTIRILMASI]

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dc.contributor.authorArzuk E.
dc.contributor.authorAlbayrak G.
dc.contributor.authorErgüç A.
dc.contributor.authorAtiş E.
dc.contributor.authorTan İ.
dc.contributor.authorBaykan Ş.
dc.date.accessioned2024-08-31T07:42:10Z
dc.date.available2024-08-31T07:42:10Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: This study aims to investigate the anticancer potential of Prangos Heyniae H. Duman & M. F. Watson root extracts against human hepatoma cells, and examine the molecular mechanisms potentially involved in extract-induced cytotoxicity. Material and Method: HepG2 cells were treated with chloroform, n-hexane, or methanol extracts from roots of P. heyniae to investigate the possible effects on cell viability. Following the determination of IC50 values by the MTT test, n-hexane, and methanol extracts were excluded because of their selectivity indices. The chemical characterization of chloroform extract was performed by HPLC to understand the chemical composition-bioactivity relationship. Alterations induced by chloroform extract on mitochondrial membrane potential and caspase-3 activation were further investigated. In addition, cell viability was measured in the presence of different selective inhibitors of pathways to define the type of cell death pathway contributing to cytotoxicity. Result and Discussion: Chloroform extract but not n-hexane or methanol extracts led to strong and selective inhibition of cell viability on HepG2 cells. In addition, cytotoxicity increased by chloroform extract was only restored in the presence of a pan-caspase apoptosis inhibitor. Also, treatment of HepG2 cells with chloroform extract impaired mitochondrial membrane potential and led to significant caspase-3 activation. Oxypeucedanin, isoimperatorin, and osthole were detected as the major components of the chloroform extract. These results represent that apoptosis may be involved in the anticancer effect of coumarin and furanocoumarin derivatives in chloroform extract. © 2024 University of Ankara. All rights reserved.en_US
dc.identifier.doi10.33483/jfpau.1336857
dc.identifier.endpage55en_US
dc.identifier.issn2564-6524
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85184781724en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage46en_US
dc.identifier.trdizinid1222539en_US
dc.identifier.urihttps://doi.org/10.33483/jfpau.1336857
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1222539
dc.identifier.urihttps://hdl.handle.net/11454/103764
dc.identifier.volume48en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherUniversity of Ankaraen_US
dc.relation.ispartofAnkara Universitesi Eczacilik Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectAnticancer effecten_US
dc.subjectapoptosisen_US
dc.subjectliver canceren_US
dc.subjectPrangos heyniaeen_US
dc.titleINVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS; [PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EKSTRELERİNİN HEPG2 HÜCRELERİNDEKİ SİTOTOKSİK VE APOPTOTİK ETKİLERİNİN ARAŞTIRILMASI]en_US
dc.typeArticleen_US

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