Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design
dc.contributor.author | Ece, Selin | |
dc.contributor.author | Evran, Serap | |
dc.contributor.author | Janda, Jan-Oliver | |
dc.contributor.author | Merkl, Rainer | |
dc.contributor.author | Sterner, Reinhard | |
dc.date.accessioned | 2019-10-27T23:00:18Z | |
dc.date.available | 2019-10-27T23:00:18Z | |
dc.date.issued | 2015 | |
dc.department | Ege Üniversitesi | en_US |
dc.description.abstract | Neopullulanase, a glycosyl hydrolase from Bacillus stearothermophilus (bsNpl), is a potentially valuable enzyme for starch and detergent industries. However, as the protein is not active at elevated temperatures and high surfactant concentrations, we aimed to increase its stability by rational enzyme design. Nine potentially destabilizing cavities were identified in the crystal structure of the enzyme. Based on computational predictions, these cavities were filled by residues with bulkier side chains. The five Asp46Glu, Val239Leu, Val404Leu, Ser407Thr and Ala566Leu exchanges resulted in a drastic stabilization of bsNpl against inactivation by heat and detergents. The catalytic activity of the variants was identical to the wild-type enzyme. | en_US |
dc.description.sponsorship | TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [210T074]; BMBFFederal Ministry of Education & Research (BMBF) [01DL12022] | en_US |
dc.description.sponsorship | This work was supported by a joint Turkish-German collaboration project of TUBITAK (IntenC project number 210T074) and BMBF (project number 01DL12022). | en_US |
dc.identifier.doi | 10.1093/protein/gzv001 | en_US |
dc.identifier.endpage | 151 | en_US |
dc.identifier.issn | 1741-0126 | |
dc.identifier.issn | 1741-0134 | |
dc.identifier.issue | 6 | en_US |
dc.identifier.pmid | 25680359 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.startpage | 147 | en_US |
dc.identifier.uri | https://doi.org/10.1093/protein/gzv001 | |
dc.identifier.uri | https://hdl.handle.net/11454/51976 | |
dc.identifier.volume | 28 | en_US |
dc.identifier.wos | WOS:000358017700001 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford Univ Press | en_US |
dc.relation.ispartof | Protein Engineering Design & Selection | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | enzyme design | en_US |
dc.subject | neopullulanase | en_US |
dc.subject | surfactant stability | en_US |
dc.subject | thermal stability | en_US |
dc.title | Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design | en_US |
dc.type | Article | en_US |