Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design

dc.contributor.authorEce, Selin
dc.contributor.authorEvran, Serap
dc.contributor.authorJanda, Jan-Oliver
dc.contributor.authorMerkl, Rainer
dc.contributor.authorSterner, Reinhard
dc.date.accessioned2019-10-27T23:00:18Z
dc.date.available2019-10-27T23:00:18Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractNeopullulanase, a glycosyl hydrolase from Bacillus stearothermophilus (bsNpl), is a potentially valuable enzyme for starch and detergent industries. However, as the protein is not active at elevated temperatures and high surfactant concentrations, we aimed to increase its stability by rational enzyme design. Nine potentially destabilizing cavities were identified in the crystal structure of the enzyme. Based on computational predictions, these cavities were filled by residues with bulkier side chains. The five Asp46Glu, Val239Leu, Val404Leu, Ser407Thr and Ala566Leu exchanges resulted in a drastic stabilization of bsNpl against inactivation by heat and detergents. The catalytic activity of the variants was identical to the wild-type enzyme.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [210T074]; BMBFFederal Ministry of Education & Research (BMBF) [01DL12022]en_US
dc.description.sponsorshipThis work was supported by a joint Turkish-German collaboration project of TUBITAK (IntenC project number 210T074) and BMBF (project number 01DL12022).en_US
dc.identifier.doi10.1093/protein/gzv001en_US
dc.identifier.endpage151en_US
dc.identifier.issn1741-0126
dc.identifier.issn1741-0134
dc.identifier.issue6en_US
dc.identifier.pmid25680359en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org/10.1093/protein/gzv001
dc.identifier.urihttps://hdl.handle.net/11454/51976
dc.identifier.volume28en_US
dc.identifier.wosWOS:000358017700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofProtein Engineering Design & Selectionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectenzyme designen_US
dc.subjectneopullulanaseen_US
dc.subjectsurfactant stabilityen_US
dc.subjectthermal stabilityen_US
dc.titleImproving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme designen_US
dc.typeArticleen_US

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