Prenatal Dexamethasone Exposure in Male Rats Alters Gene Expression Patterns of Epigenetic Enzymes in Hippocampus and Cortex

Basit Öğe Kaydı
dc.authoridUyanikgil, Yigit/0000-0002-4016-0522
dc.authorwosidUyanikgil, Yigit/M-2746-2019
dc.contributor.authorTurunc, Ezgi
dc.contributor.authorUyanikgil, Yigit
dc.contributor.authorTemiz, Tijen Kaya
dc.contributor.authorYalcin, Ayfer
dc.date.accessioned2023-01-12T20:18:43Z
dc.date.available2023-01-12T20:18:43Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractObjective: This study aimed to examine the effects of prenatal stress (PS) induced by dexamethasone exposure on gene expression levels of epigenetic enzymes in hippocampus and cerebral cortex of male rats through relative mRNA levels of histone acetyltransferases (activating transcription factor 2, P300), histone deacetylases (HDAC1, HDAC2), and DNA methyltransferases (DNMT1, DNMT3a, DNMT3b). Methods: Pregnant rats were daily injected subcutaneously with dexamethasone (0.2 mg/kg) or saline during the third week of gestation. After birth, male rats were killed at 90 days of age (n = 5 for control and dexamethasone groups). Hippocampal and cortical tissues were used for gene expression analyses. The effects of dexamethasone on epigenetic mechanisms were investigated by real-time polymerase chain reaction through relative mRNA levels of DNMT1, DNMT3a, DNMT3b, activating transcription factor 2, P300, HDAC1, and HDAC2. Statistical comparisons were performed with Student's t-test. Results: Prenatal dexamethasone exposure (PDE) caused increased DNMT1, DNMT3a, DNMT3b, activating transcription factor 2 and decreased P300 mRNA levels in hippocampus while increased DNMT3a, DNMT3b, activating transcription factor 2, P300, HDAC1, and HDAC2 mRNA levels were achieved in cortex. Furthermore, no significant differences were obtained in cortical DNMT1 and hippocampal HDAC1 and HDAC2 gene expression levels between control and prenatally stressed rats. Conclusion: Our results emphasize the effect of prenatal dexamethasone exposure on gene expression levels of epigenetic enzymes involved in histone acetylation/deacetylation and DNA methylation in male rats and suggest that prenatal stress may lead to epigenetic dysregulation through alterations in hippocampal and cortical gene expression patterns of DNMT1, DNMT3a, DNMT3b, activating transcription factor 2, P300, HDAC1, and HDAC2.en_US
dc.description.sponsorshipIzmir Katip Celebi University [2018-ONAP-ECZF-0001]en_US
dc.description.sponsorshipThis study was supported by a grant from Izmir Katip Celebi University (2018-ONAP-ECZF-0001en_US
dc.identifier.doi10.5152/EurJTher.2022.22056
dc.identifier.endpage225en_US
dc.identifier.issn2564-7784
dc.identifier.issn2564-7040
dc.identifier.issue3en_US
dc.identifier.startpage219en_US
dc.identifier.trdizinid1131763en_US
dc.identifier.urihttps://doi.org/10.5152/EurJTher.2022.22056
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1131763
dc.identifier.urihttps://hdl.handle.net/11454/78887
dc.identifier.volume28en_US
dc.identifier.wosWOS:000865982700010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofEuropean Journal Of Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDexamethasoneen_US
dc.subjectepigenetic enzymesen_US
dc.subjectprenatal stressen_US
dc.subjectraten_US
dc.subjectBdnf Expressionen_US
dc.subjectStressen_US
dc.subjectMelatoninen_US
dc.subjectModelen_US
dc.subjectPcren_US
dc.titlePrenatal Dexamethasone Exposure in Male Rats Alters Gene Expression Patterns of Epigenetic Enzymes in Hippocampus and Cortexen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu