Label-free highly sensitive detection of DNA approximate length and concentration by impedimetric CRISPR-dCas9 based biosensor technology

Basit Öğe Kaydı
dc.authoriduygun, zihni onur/0000-0001-9045-7271
dc.authorwosiduygun, zihni onur/P-8783-2015
dc.contributor.authorUygun, Zihni Onur
dc.contributor.authorAtay, Sevcan
dc.date.accessioned2023-01-12T19:54:28Z
dc.date.available2023-01-12T19:54:28Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractIn this study, we designed a CRISPR-dCas9-based biosensor with potential clinical use in glioblastoma subtype discrimination through detection of isocitrate dehydrogenase R132H (IDH) mutation status. The electrode was modified to detect mutant DNA cysteamine (Cys), PAMAM, dCas9 and sgRNA for R132H mutations, respectively. The biosensor system we proposed was able not only to detect mutant DNA, but also to measure the approximate length of DNA. Therefore, it can be considered that the biosensor technology that we developed is novel in the field of DNA biosensors. Another superior capability of the biosensor system is that it can simultaneously measure DNA concentration by electrochemical impedance spectroscopy and DNA length by capacitive detection, which lowers the concentration-based false positive signals. The calibration range was obtained between 100 fM and 1000 fM, LOD and LOQ were also calculated as 33.96 fM and 102.91 fM respectively. Moreover, thanks to the sensitivity of the capacitive detection, the biosensor was able to discriminate the same EIS signals of the 200 bp and 250 fM concentration data and 1000 bp and 50 fM concentration data. In conclusion, the biosensor was capable of detect target DNA and DNA length, simultaneously in minutes. (c) 2021 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination [TGA-2020-22230]en_US
dc.description.sponsorshipAuthors are thankful by support of Ege University Scientific Research Projects Coordination (TGA-2020-22230).en_US
dc.identifier.doi10.1016/j.bioelechem.2021.107812
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid33845443en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2021.107812
dc.identifier.urihttps://hdl.handle.net/11454/76430
dc.identifier.volume140en_US
dc.identifier.wosWOS:000663599600004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectChronoimpedanceen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectCapacitanceen_US
dc.subjectCRISPRen_US
dc.subjectdCas9en_US
dc.subjectIdh2 Mutationsen_US
dc.subjectGlioblastomaen_US
dc.subjectPolypyrroleen_US
dc.subjectSurvivalen_US
dc.titleLabel-free highly sensitive detection of DNA approximate length and concentration by impedimetric CRISPR-dCas9 based biosensor technologyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu