Akut İshali Olan Hastalarda Rotavirüs ve Norovirüs Sıklığının Araştırılması
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Giriş: Bu çalışmada, yaklaşık bir yıllık süreçte bir üniversite hastanesine akut ishal şikayetiyle ayaktan başvuran veya akut gastroenterit ön tanısıyla hastanede yatarak izlenen hastalardaki rotavirüs ve norovirüs sıklığının araştırılması amaçlanmıştır. Materyal ve Metod: Eylül 2016-Ağustos 2017 tarihleri arasında akut gastroenterit ön tanısıyla hastaneye başvuran hastalardan laboratuvara gönderilen 408 dışkı örneği incelenmiştir. Bu örnekler, yaş aralığı bir ay ile 84 yaş arasında değişen (medyan: 3.0 yaş, ortalama: 10.25 yaş), 341 (%83.5)’i çocuk, 67 (%16.4)’si yetişkin hastalardan toplanmıştır. Dışkı örneklerinin 206 (%50.5)’sı polikliniğe başvuran, 202 (%49.5)’si yatan hastalardan elde edilmiştir. Akut gastroenterit ön tanılı hastalardan toplanan dışkı örnekleri norovirüs (genotip I ve II) ve rotavirüs türlerini kalitatif olarak saptayan otomatize ve kapalı bir sistem olan multipleks gerçek zamanlı polimeraz zincir reaksiyonu (rt-PCR) testi (BD MAX™ Enteric Viral Panel, BD Diagnostics, Baltimore, MD, USA) ile incelenmiştir. Bu multipleks PCR sisteminde örnekte bulunan viral nükleik asitler kapalı ve otomatize şekilde ekstrakte edilip, revers transkripsiyonla komplementer DNA (cDNA) sentezlenip, hedef cDNA rt-PCR yöntemiyle amplifiye edildikten sonra virüsler kalitatif olarak saptanmıştır. Bulgular: Akut gastroenterit şikayetiyle başvuran 408 hastanın 223 (%54.7)’ü en az bir virüs açısından pozitif bulunmuştur. Pozitif örneklerin 184 (%45.1)’ünde rotavirüs, 26 (%6.4)’sında norovirüs genotip II, 1 (%0.2)’inde norovirüs genotip I ve 12 (%2.9)’sinde rotavirüs ve norovirüs genotip II birlikte saptanmıştır. Pozitif bulunan örneklerin 121 (%54.3)’i poliklinik hastalarından, 102 (%45.7)’si yatan hastalardan gönderilmiştir (p= 0.094). Rotavirüs için pozitiflik oranı pediatrik ve yetişkin hasta grupları için sırasıyla %48.4 (165/341) ve %46.3 (31/67) olarak saptanmıştır (p= 0.751). Rotavirüs pozitifliğinin en çok mart (%75), şubat (%70) ve ocak (%60) aylarında olduğu görülmüştür. Sonuç: Akut viral gastroenterit ön tanısıyla laboratuvara gönderilen dışkı örneklerinde özellikle kış aylarında rotavirüs pozitifliği yaklaşık %48 oranında, norovirüs pozitifliği ise yaklaşık %10 oranında bulunmuştur. Yatan ya da ayaktan hastaların pozitiflik oranlarında ve çocuk ya da erişkin hastaların pozitiflik oranlarında anlamlı bir fark bulunamamıştır
Introduction: in this study, it was aimed to investigate the prevalence of rotavirus and norovirus in patients admitted to Ege University Medical Faculty Hospital with acute diarrhea in a period of about one year. Materials and Methods: Between September 2016-August 2017, stool specimens were collected from 408 (341 pediatric and 67 adult) patients with acute diarrhea. the age range of the patients was between one month to 84 years (median: 3 years, mean: 10.25 years). Stool specimens were obtained from 206 (50.5%) outpatients and 202 (49.5%) inpatients. Stool specimens of patients with acute gastroenteritis were tested by real time PCR BD MAX™ Enteric Viral Panel (BD Diagnostics, Baltimore, MD, USA). This is an automated in vitro diagnostic test used for the qualitative detection of norovirus (genotype I and II) and rotavirus species in stool specimens. in this multiplex PCR system, viral nucleic acid is extracted from the specimen and it undergoes reverse transcription to generate complementary DNA (cDNA). the target cDNA is amplified using real time PCR and then analyzed to detect the presence or absence of each virus in the panel. Results: of the 408 specimens tested, 223 (54.7%) were positive for one or more gastrointestinal viruses. of the 223 positive samples, 184 (45.1%) were rotavirus, 26 (6.4%) were norovirus genotype II, 1 (0.2%) was norovirus genotype I, and 12 (2.9%) were rotavirus/ norovirus genotype II. of the 223 positive samples, 121 (54.3%) were outpatients and 102 (45.7%) were inpatients (p= 0.094). the rate of positivity for the rotavirus in pediatric and adult groups were 48.4% (165/341) and 46.3% (31/67) (p= 0.751), respectively. Rotavirus positivity was highest in the months of March (75%), February (70%) and January (60%). Conclusion: in conclusion, the rate of positivity for rotavirus and norovirus were found as 48% and 10%, respectively in fecal samples sent to the laboratory by viral acute gastroenteritis, especially in winter. There was no significant difference between the positivity rates of the inpatients or outpatients and the positivity rates of the pediatric or adult patients.
Introduction: in this study, it was aimed to investigate the prevalence of rotavirus and norovirus in patients admitted to Ege University Medical Faculty Hospital with acute diarrhea in a period of about one year. Materials and Methods: Between September 2016-August 2017, stool specimens were collected from 408 (341 pediatric and 67 adult) patients with acute diarrhea. the age range of the patients was between one month to 84 years (median: 3 years, mean: 10.25 years). Stool specimens were obtained from 206 (50.5%) outpatients and 202 (49.5%) inpatients. Stool specimens of patients with acute gastroenteritis were tested by real time PCR BD MAX™ Enteric Viral Panel (BD Diagnostics, Baltimore, MD, USA). This is an automated in vitro diagnostic test used for the qualitative detection of norovirus (genotype I and II) and rotavirus species in stool specimens. in this multiplex PCR system, viral nucleic acid is extracted from the specimen and it undergoes reverse transcription to generate complementary DNA (cDNA). the target cDNA is amplified using real time PCR and then analyzed to detect the presence or absence of each virus in the panel. Results: of the 408 specimens tested, 223 (54.7%) were positive for one or more gastrointestinal viruses. of the 223 positive samples, 184 (45.1%) were rotavirus, 26 (6.4%) were norovirus genotype II, 1 (0.2%) was norovirus genotype I, and 12 (2.9%) were rotavirus/ norovirus genotype II. of the 223 positive samples, 121 (54.3%) were outpatients and 102 (45.7%) were inpatients (p= 0.094). the rate of positivity for the rotavirus in pediatric and adult groups were 48.4% (165/341) and 46.3% (31/67) (p= 0.751), respectively. Rotavirus positivity was highest in the months of March (75%), February (70%) and January (60%). Conclusion: in conclusion, the rate of positivity for rotavirus and norovirus were found as 48% and 10%, respectively in fecal samples sent to the laboratory by viral acute gastroenteritis, especially in winter. There was no significant difference between the positivity rates of the inpatients or outpatients and the positivity rates of the pediatric or adult patients.
Açıklama
Anahtar Kelimeler
Enfeksiyon Hastalıkları, Mikrobiyoloji
Kaynak
Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
24
Sayı
2
