Distribution and Phylogenetic Analysis of Subtypes and Alleles of Blastocystis sp. in the Stool Samples Collected from Patients with Gastrointestinal Complaints in Izmir, Turkey

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dc.authoridAykur, Mehmet/0000-0002-6100-1037
dc.authorscopusid55334665700
dc.authorscopusid57200799646
dc.authorscopusid35795253800
dc.authorscopusid57212299393
dc.authorscopusid6506677021
dc.authorscopusid8905840200
dc.authorscopusid6507143975
dc.authorwosidAykur, Mehmet/I-2963-2012
dc.contributor.authorAykur, Mehmet
dc.contributor.authorKurt, Cansu Caliskan
dc.contributor.authorErdogan, Derya Dirim
dc.contributor.authorBiray Avci, Cigir
dc.contributor.authorVardar, Rukiye
dc.contributor.authorAydemir, Sohret
dc.contributor.authorGirginkardesler, Nogay
dc.date.accessioned2024-08-25T18:38:58Z
dc.date.available2024-08-25T18:38:58Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractPurposeBlastocystis sp. is one of the most prevalent intestinal protozoa found in humans and many other animals. The present study aimed to examine the distribution and genetic diversity of Blastocystis sp. in stool samples from patients with gastrointestinal complaints in Izmir, Turkey.MethodsAll stool samples of 439 patients with gastrointestinal complaints were examined by native-Lugol and trichrome staining. To investigate the presence of Blastocystis sp. in stool samples, DNA was isolated, and PCR was performed with the barcode region in the SSU rRNA gene. PCR positive samples were sequenced to identify subtypes and alleles of Blastocystis sp.ResultsThe prevalence of Blastocystis sp. was found to be 16.6% (73/439) in patients with gastrointestinal complaints in Izmir, Turkey. Three different Blastocystis sp. subtypes were identified. ST3 (28/55; 51.0%) was the most common subtype followed by ST2 (19/55; 34.5%) and ST1 (8/55; 14.5%). Itching and diarrhea were the most prominent clinical symptoms in Blastocystis sp. positive patients. When clinical symptoms and subtypes were compared, diarrhea was found in 62.5%, 47.4%, and 46.4% of patients with ST1, ST2, and ST3 subtypes, respectively. In addition, itching was found in 37.5%, 32.1%, and 21.1% of patients with ST1, ST3, and ST2, respectively. Six distinct alleles were identified by allele analysis of Blastocystis 18S rRNA gene: allele 4 for ST1, alleles 9, 11, and 12 for ST2, and alleles 34 and 36 for ST3. In this study, Blastocystis sp. was detected in 16 of 21 districts, including the central and rural districts of Izmir. Although ST1 was detected in central districts, it was not found in rural districts.ConclusionThis study provides comprehensive data on the prevalence and molecular epidemiology of the genetic diversity at the level of subtypes and alleles of Blastocystis sp. in different districts of Izmir province in Turkey. To the best of our knowledge, this is the first study which evaluates the distribution of subtypes and alleles of Blastocystis sp. according to PCR and SSU rRNA gene sequencing in patients with gastrointestinal complaints in different districts of Izmir province in Turkey.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, Turkey [13-TIP-092]en_US
dc.description.sponsorshipAcknowledgementsWe thank all the patients who participated in the study. This study was partly supported by the grant given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant number: 13-TIP-092).en_US
dc.identifier.doi10.1007/s11686-023-00665-2
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.pmid36806111en_US
dc.identifier.scopus2-s2.0-85148512518en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1007/s11686-023-00665-2
dc.identifier.urihttps://hdl.handle.net/11454/101208
dc.identifier.wosWOS:000935456300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Int Publ Agen_US
dc.relation.ispartofActa Parasitologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectBlastocystis spen_US
dc.subjectSubtypingen_US
dc.subjectAllelesen_US
dc.subjectPatientsen_US
dc.subjectGastrointestinal complaintsen_US
dc.subjectIzmiren_US
dc.subjectTurkeyen_US
dc.subjectMolecular Characterizationen_US
dc.subjectGeographic-Distributionen_US
dc.subjectClinical-Significanceen_US
dc.subjectRisk-Factorsen_US
dc.subjectHominisen_US
dc.subjectDiversityen_US
dc.subjectProvinceen_US
dc.subjectWateren_US
dc.subjectSpp.en_US
dc.subjectIdentificationen_US
dc.titleDistribution and Phylogenetic Analysis of Subtypes and Alleles of Blastocystis sp. in the Stool Samples Collected from Patients with Gastrointestinal Complaints in Izmir, Turkeyen_US
dc.typeArticleen_US

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