Electrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodes

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dc.contributor.authorTurunc, Ezgi
dc.contributor.authorKaradeniz, Hakan
dc.contributor.authorArmagan, Guliz
dc.contributor.authorErdem, Arzum
dc.contributor.authorYalcin, Ayfer
dc.date.accessioned2019-10-27T22:07:27Z
dc.date.available2019-10-27T22:07:27Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractGlutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found to be 0.47 mu M (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R-2=0.976).en_US
dc.description.sponsorshipTUBATurkish Academy of Sciences; Technological and Scientific Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipA.E., an associate member of Turkish Academy of Sciences (TUBA), expresses her gratitude to the TUBA for its partial support. E. T. was supported by doctoral grant from the Technological and Scientific Council of Turkey (TUBITAK).en_US
dc.identifier.endpage701en_US
dc.identifier.issn1386-2073
dc.identifier.issn1875-5402
dc.identifier.issue9en_US
dc.identifier.pmid23782036en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage695en_US
dc.identifier.urihttps://hdl.handle.net/11454/49015
dc.identifier.volume16en_US
dc.identifier.wosWOS:000327439000003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofCombinatorial Chemistry & High Throughput Screeningen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDifferential pulse voltammetryen_US
dc.subjectglutathioneen_US
dc.subjectmulti-walled carbon nanotubesen_US
dc.subjectplasma samplesen_US
dc.subjectscreen-printed electrodesen_US
dc.subjectspectrophotometryen_US
dc.titleElectrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodesen_US
dc.typeArticleen_US

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