Bioapplications of Polythiophene-g-Polyphenylalanine-Covered Surfaces

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dc.contributor.authorGuler E.
dc.contributor.authorAkbulut H.
dc.contributor.authorBozokalfa G.
dc.contributor.authorDemir B.
dc.contributor.authorEyrilmez G.O.
dc.contributor.authorYavuz M.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorCoskunol H.
dc.contributor.authorEndo T.
dc.contributor.authorYamada S.
dc.contributor.authorTimur S.
dc.contributor.authorYagci Y.
dc.date.accessioned2019-10-26T21:21:53Z
dc.date.available2019-10-26T21:21:53Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThe fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of "integrin ?vß3" receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode. Thiophene-functionalized polyphenylalanine macromonomer is synthesized and used as polymerized form "Polythiophene-graft-polyphenylalanine" in electrochemical biosensing and cell adhesion applications. Initially, it is conducted to glucose sensing via glucose oxidase immobilization, and secondly the constructed surfaces on indium tin oxide within the modification of RGD peptide are used for the selective cell binding in the way of cell viability and electrochemical biosensing platform. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.identifier.doi10.1002/macp.201500219
dc.identifier.endpage1878en_US
dc.identifier.issn1022-1352
dc.identifier.issn1022-1352en_US
dc.identifier.issue18en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1868en_US
dc.identifier.urihttps://doi.org/10.1002/macp.201500219
dc.identifier.urihttps://hdl.handle.net/11454/16917
dc.identifier.volume216en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-VCH Verlagen_US
dc.relation.ispartofMacromolecular Chemistry and Physicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcell adhesionen_US
dc.subjectelectropolymerizationen_US
dc.subjectgraft copolymeren_US
dc.subjectpolypeptide macromonomeren_US
dc.subjectsurface designen_US
dc.titleBioapplications of Polythiophene-g-Polyphenylalanine-Covered Surfacesen_US
dc.typeArticleen_US

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