Bioapplications of Polythiophene-g-Polyphenylalanine-Covered Surfaces
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-VCH Verlag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of "integrin ?vß3" receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode. Thiophene-functionalized polyphenylalanine macromonomer is synthesized and used as polymerized form "Polythiophene-graft-polyphenylalanine" in electrochemical biosensing and cell adhesion applications. Initially, it is conducted to glucose sensing via glucose oxidase immobilization, and secondly the constructed surfaces on indium tin oxide within the modification of RGD peptide are used for the selective cell binding in the way of cell viability and electrochemical biosensing platform. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Açıklama
Anahtar Kelimeler
cell adhesion, electropolymerization, graft copolymer, polypeptide macromonomer, surface design
Kaynak
Macromolecular Chemistry and Physics
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
216
Sayı
18
