Leukotrienes in cerulein-induced acute pancreatitis in rats and effect of octreotide
Küçük Resim Yok
Tarih
1999
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The role of leukotrienes (LTs) in inflammation, which is widespread in some diseases, suggests that LTs may play an important role in the pathophysiology of acute pancreatitis (AP). The aim of this study was to investigate the levels of LT in the liver, pancreatic tissue and plasma of rats with cerulein-induced acute pancreatitis and the effect of octreotide on these levels. Methods: Thirty three Wistar Albino rats (170-220g) were included in the study, and randomly allocated to 5 groups (n=6-7) which were given: A-Control, BI: four successive doses of cerulein, 20 µg/kg SC at one hourly intervals. BII: Same as group CI, with addition of four successive doses of octreoide, 5 µg/kg at one hourly intervals. CI: Same as group BI with the addition of saline given every 12 hr for 1 wk CII-Same as group IV, but with 5 µg/kg octreotide given instead of saline. LTs were measured by RAI method. Results: these were expressed as (mean±SEM) pg/mg tissue protein for liver and pancreas and pg/ml for plasma. Plasma, pancreas and liver LTB4 levels were: 15.48±4.03, 13.51±2.99 and 33.42±6.01 in group A; 32.44±2.89, 32.71±3.24 and 37.42±2.29 in group BI; 18.21±2.67, 6.78±1.69 and 23.42±3.56 in group BII; 26.03±2.53, 96.66±13.22 and 38.50±5.69 in group CI; 20.0±1.75, 61.5±2.14 and 27.83±1.35 in group CII respectively. Plasma, pancreas and liver LTC4 levels were found 20.71±6.99, 22.57±6.76 and 11.14±5.44 in group A; 31.85±3.37, 51.14±13.92 and 21.14±4.29 in group BI; 10.14±2.91, 31.57±10.11, 13.14±2.65 in group BII; 29.00±3.69, 84.00±17.47 and 30.83±2.42 in group CI and 19.16±1.90, 59.66±11.72 and 9.83±4.50 in group CII respectively. Plasma LTB4 levels were significantly different between group A and group BI (p<0.01), pancreatic LTB4 levels were significantly different between group A: and groups BI, CI and CII (p<0.01), group BII plasma LTC4 levels was significantly lower then group BI (p<0.01) and pancreas and liver LTC4 levels were significantly different between groups A and CI (p<0.05). Conclusion: In the acute phase of acute pancreatitis, LTB4 levels increase in plasma and pancreatic tissue. Octreotide decreases pancreatic and liver tissue LTB4 levels. One week after induction of acute pancreatitis, LTC4 increases significantly in the liver and pancreas and this increase is inhibited by octreotide. LTs play an important role in the pathogenesis of cerulein-induced acute pancreatitis in rats.
Açıklama
Anahtar Kelimeler
Leukotriene B4, Leukotriene C4, Liver injury, Octreotide, Pancreatitis
Kaynak
Turkish Journal of Gastroenterology
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
10
Sayı
1
