Synthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNA

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dc.authoridErdem, Arzum/0000-0002-4375-8386
dc.authoridButun Sengel, Sultan/0000-0001-7036-2224
dc.contributor.authorTunca, Nilay
dc.contributor.authorMaral, Meltem
dc.contributor.authorYildiz, Esma
dc.contributor.authorSengel, Sultan Butun
dc.contributor.authorErdem, Arzum
dc.date.accessioned2024-08-31T07:50:05Z
dc.date.available2024-08-31T07:50:05Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractThe main goal of our study is to demonstrate the applicability of the PPy-cryogel-modified electrodes for electrochemical detection of DNA. First, a polysaccharide-based cryogel was synthesized. This cryogel was then used as a template for chemical polypyrrole synthesis. This prepared polysaccharide-based conductive cryogel was used for electrochemical biosensing on DNA. Carrageenan (CG) and sodium alginate (SA) polysaccharides, which stand out as biocompatible materials, were used in cryogel synthesis. Electron transfer was accelerated by polypyrrole (PPy) synthesized in cryogel networks. A 2B pencil graphite electrode with a diameter of 2.00 mm was used as a working electrode. The prepared polysaccharide solution was dropped onto a working electrode as a support material to improve the immobilization capacity of biomolecules and frozen to complete the cryogelation step. PPy synthesis was performed on the electrodes whose cryogelation process was completed. In addition, the structures of cryogels synthesized on the electrode surface were characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Surface characterization of the modified electrodes was performed by energy-dispersive X-ray spectroscopy (EDX) analysis. Electrochemical determination of fish sperm DNA (fsDNA) was performed using a PPy-cryogel-modified electrode. The use of a porous 3D cryogel intermediate material enhanced the signal by providing a large surface area for the synthesis of PPy and increasing the biomolecule immobilization capacity. The detection limit was 0.98 mu g mL(-1) in the fsDNA concentration range 2.5-20 mu g mL(-1). The sensitivity of the DNA biosensor was estimated to 14.8 mu A mM(-1) cm(-2). The stability of the biosensor under certain storage conditions was examined and observed to remain 66.95% up to 45 days.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK)en_US
dc.description.sponsorshipOpen access funding provided by the Scientific and Technological Research Council of Turkiye (TUBITAK). DAS:Not applicable.en_US
dc.identifier.doi10.1007/s00604-024-06550-7
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue8en_US
dc.identifier.pmid39088080en_US
dc.identifier.scopus2-s2.0-85200098623en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s00604-024-06550-7
dc.identifier.urihttps://hdl.handle.net/11454/105109
dc.identifier.volume191en_US
dc.identifier.wosWOS:001282887600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Wienen_US
dc.relation.ispartofMicrochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectPolypyrroleen_US
dc.subjectCryogel Modified Electrodeen_US
dc.subjectGraphite Pencil Electrodeen_US
dc.subjectPolysaccharide Cryogelen_US
dc.subjectElectrochemical Dna Biosensoren_US
dc.subjectCyclic Voltammetryen_US
dc.titleSynthesis and characterization of polysaccharide-cryogel and its application to the electrochemical detection of DNAen_US
dc.typeArticleen_US

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