İkinci kuşak purified vero rabies vaccine (PVRV) kuduz aşısının sağlıklı gönüllülerdeki etkinliğinin araştırılması
Küçük Resim Yok
Tarih
1996
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Araştırma, ikinci kuşak kuduz aşılarından Purified Vero Rabies Vaccine (PVRV)'nin sağlıklı gönüllülerdeki etkinliğini ortaya koymak için yapılmıştır. Daha önce kuduz aşısı ve serumu yapılmamış Ege Üniversitesi Tıp Fakültesi öğrencilerinden 38, hastane personelinden iki kişi olmak üzere toplam 40 kişi üzerinde denenmiştir. Sıfır, yedi ve 21. günlerde 2+1+1 şemasına uygun olarak ilk gün sağ ve sol deltoit kastan iki tane, yedi ve 21. günlerde birer tane olmak üzere toplam dört tane aşı uygulanmıştır. Sıfır, yedi ve 21. günlerde ve aşılamadan önce 28. ve 90. günlerde deneklerin serumları alınarak -40°C'de saklanmış ve daha sonra ELİSA ile nötralizan antikor titreleri araştırılmıştır. Sıfır ve yedinci günlerdeki serumlarda koruyucu antikor titreleri Dünya Sağlık Örgütü'nün önerdiği 0.5 ILJ/ml'nin üzerinde bulunmamıştır; 21.-28.-90. günlerdeki serumlarda birisi dışında tümünde yüksek düzeyde antikor titreleri saptanmıştır. Bu değerler istatistiksel olarak anlamlı bulunmuştur. Tüberkülin testi olumsuz olan tek olguda 21, 28 ve 90. günlerde sırasıyla 0.7, 0.7 ve 0.5 ILJ/ml limitte antikor düzeyi saptanmıştır. Uygulama süresi, sayısı ve maliyeti açısından avanatajlı olan ve beş-altı doz olarak kullanılan Human Diploid Cell Vaccine (HDCV) kadar etkili koruyucu antikor düzeyi sağlayan PVRV'nin Türkiye'de de kullanılabileceği sonucuna varılmıştır.
The aim of this study was to evaluate the effectiveness of PVRV which is a second generation rabies vaccine on healthy volunteers. Thirty-eight students from Ege University Medical School and two medical personnel who had not been vaccinated with rabies vaccine or treated with rabies antiserum prioriiy, were selected for the study. According to the 2+1+1 scheme on days 0, 7 and 21 totally four doses vaccine were given appropriately to each volunteer (first day two vaccines were given to each deltoid muscle and on days 7 and 21 only one vaccine dose was given). the serum of the volunteers were collected on days 0,7 and 21 before vaccination and on days 28 and 90 after vaccination. Sera were stored at -40°C until use. the neutralizing antibody titers were determined with ELISA. on days 0 and 7 the protecting antibody levels were found under the level of 0.5 lU/ml which is the recommended limit by WHO. But on days 21, 28 and 90 high levels of antibody titers were detected in all sera of volunteers except in one .sample. These values were statistically significant. in one case who was tuberculin-negative the antibody levels were 0.7, 0.7 and 0.5 lU/ml on days 21, 28 and 90, respectively. These values are considered as borderline values. It is shown that PVRV is as effective as Human Diploid Cell Vaccine (HDCV): the vaccination period, number of vaccine doses and cost of vaccination are advantages of PVRV. It is concluded that PVRV can Be used in Turkey.
The aim of this study was to evaluate the effectiveness of PVRV which is a second generation rabies vaccine on healthy volunteers. Thirty-eight students from Ege University Medical School and two medical personnel who had not been vaccinated with rabies vaccine or treated with rabies antiserum prioriiy, were selected for the study. According to the 2+1+1 scheme on days 0, 7 and 21 totally four doses vaccine were given appropriately to each volunteer (first day two vaccines were given to each deltoid muscle and on days 7 and 21 only one vaccine dose was given). the serum of the volunteers were collected on days 0,7 and 21 before vaccination and on days 28 and 90 after vaccination. Sera were stored at -40°C until use. the neutralizing antibody titers were determined with ELISA. on days 0 and 7 the protecting antibody levels were found under the level of 0.5 lU/ml which is the recommended limit by WHO. But on days 21, 28 and 90 high levels of antibody titers were detected in all sera of volunteers except in one .sample. These values were statistically significant. in one case who was tuberculin-negative the antibody levels were 0.7, 0.7 and 0.5 lU/ml on days 21, 28 and 90, respectively. These values are considered as borderline values. It is shown that PVRV is as effective as Human Diploid Cell Vaccine (HDCV): the vaccination period, number of vaccine doses and cost of vaccination are advantages of PVRV. It is concluded that PVRV can Be used in Turkey.
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Cilt
10
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4
