Coculture model of blood-brain barrier on electrospun nanofibers

Basit Öğe Kaydı
dc.contributor.authorÖztürk, Şükrü
dc.contributor.authorÖzyurt, Mustafa Görkem
dc.contributor.authorDoğan, Şule
dc.contributor.authorŞendemir, Aylin
dc.contributor.authorBayır, Ece
dc.date.accessioned2023-01-12T20:36:56Z
dc.date.available2023-01-12T20:36:56Z
dc.date.issued2020
dc.departmentN/A/Departmenten_US
dc.description.abstractThe blood–brain barrier (BBB) is a control mechanism that limits the diffusion of many substances to the central nervous system (CNS). In this study, we designed an in-vitro 3-dimensional BBB system to obtain a fast and reliable model to mimic drug delivery characteristics of the CNS. A support membrane of polycaprolactone nanofiber surfaces was prepared using electrospinning. After confirming the fiber morphology and size, endothelial cells (HUVEC) and glial cells were cultured on either side of this membrane. The model’s similarity to in vivo physiology was tested with a home-designed transmembrane resistance (TR) device, with positive and negative control molecules. Finally, 2 doses of methotrexate (MTX), a chemotherapy agent, were applied to the model, and its permeability through the model was determined indirectly by a vitality test on the MCF-7 cell line. Nicotine, the positive control, completed its penetration through the model almost instantly, while albumin, the negative control, was blocked significantly even after 2 days. MTX reached a deadly threshold 24 h after application. The TR value of the model was promising, being around 260 ohm.cm2. The provided model proposes a disposable and reliable tool for investigating drug permeability through the BBB and has the potential to reduce the number of animal experiments.en_US
dc.identifier.endpage132en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4en_US
dc.identifier.startpage121en_US
dc.identifier.trdizinid433338en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/433338
dc.identifier.urihttps://hdl.handle.net/11454/81501
dc.identifier.volume44en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmethotrexateen_US
dc.subjecttransmembrane resistanceen_US
dc.subjectelectrospinningen_US
dc.subjectBlood–brain barrieren_US
dc.subject3-dimensional cell cultureen_US
dc.titleCoculture model of blood-brain barrier on electrospun nanofibersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu