A novel method for sensitive microRNA detection: Electropolymerization based doping

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dc.contributor.authorKaplan, Merve
dc.contributor.authorKilic, Tugba
dc.contributor.authorGuler, Gunnur
dc.contributor.authorMandli, Jihane
dc.contributor.authorAmine, Aziz
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T11:07:25Z
dc.date.available2019-10-27T11:07:25Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the proposed study, for the first time, sensitive electrochemical detection of a breast cancer biomarker microRNA (miRNA), mir-21 was achieved via electropolymerized polypyrrole (PPy) modified pencil graphite electrodes (PPy/PGE). The detection of hybridization of electrochemically doped probe miRNA, antimir-21, with its complementary target, mir-21 was monitored by either electrochemical impedance spectroscopy (EIS) via comparison of charge transfer resistance (Rat) values before and after hybridization or by electrochemical reduction signal of an hybridization indicator, Meldola's blue (MDB). The study covers all the optimization steps for hybridization procedure and electropolymerization of pyrrole as well as detection from real samples of breast cancer cell line, MCF-7. The designed sensor shows a high selectivity and a low detection limit of 0.17 nM thanks to electrical conductivity and porous structure of PPy.en_US
dc.identifier.doi10.1016/j.bios.2016.09.050en_US
dc.identifier.endpage778en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid27836600en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage770en_US
dc.identifier.urihttps://doi.org/10.1016/j.bios.2016.09.050
dc.identifier.urihttps://hdl.handle.net/11454/32024
dc.identifier.volume92en_US
dc.identifier.wosWOS:000396946600105en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmicroRNAen_US
dc.subjectMir-21en_US
dc.subjectElectropolymerizationen_US
dc.subjectPyrroleen_US
dc.subjectMeldola's blueen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectDifferential pulse voltammetryen_US
dc.titleA novel method for sensitive microRNA detection: Electropolymerization based dopingen_US
dc.typeArticleen_US

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