Investigation of Dientamoeba fragilis Prevalence and Evaluation of Sociodemographic and Clinical Features in Patients with Gastrointestinal Symptoms

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dc.contributor.authorAykur, Mehmet
dc.contributor.authorKurt, Cansu Caliskan
dc.contributor.authorErdogan, Derya Dirim
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorVardar, Rukiye
dc.contributor.authorAydemir, Sohret
dc.contributor.authorGirginkardesler, Nogay
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorDagci, Hande
dc.date.accessioned2019-10-27T09:45:43Z
dc.date.available2019-10-27T09:45:43Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractBackgroundDientamoeba fragilis is a protozoan parasite of the human gastrointestinal tract and still controversial in association with gastrointestinal symptoms.PurposeWe present cross-sectional study of the prevalence of D. fragilis, and sociodemographic and clinical features in the patients with gastrointestinal symptoms.MethodsA total of 490 fecal specimens were collected from outpatients with gastrointestinal symptoms in the Department of Parasitology, Faculty of Medicine, Ege University and Celal Bayar University, Turkey. Fecal specimens were examined with microscopy and inoculated in Robinson medium. D. fragilis-positive samples were examined for the presence of other intestinal parasites using enzyme immunoassay. Real-time PCR analysis was performed on all samples.ResultsOf the 490 stool specimens examined by real-time PCR, 59 patients were positive for D. fragilis infection with prevalence rate of 12.04%. Forty-four of positive patients (74.5%) were found to be infected with only D. fragilis, while 23.7% were co-infected with Blastocystis and 1.7% were co-infected with Rotavirus. No statistically significant difference was found in all the examined patients in terms of D. fragilis positivity for all sociodemographic parameters. Loose stool consistency was associated with the presence of D. fragilis, with 18.3% (P=0.001). When the clinical symptoms of all the patients participating in this study were examined, diarrhea was statistically more significant in patients with the presence of D. fragilis (16.3%; P=0.001). The rate of diarrhea in D. fragilis-positive patients (84.09%; P=0.0005) was higher than that of D. fragilis-negative patients and it was statistically significant.ConclusionThis study is important for assessing the prevalence of D. fragilis and its association with other factors in symptomatic patients in a large sample group in Turkey, as well as investigating the relationship of identified symptoms with the D. fragilis pathogenicity. It is suggested that D. fragilis in this case is not a commensal parasite but a pathogenic parasite and that the most common clinical symptom is diarrhea.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, TurkeyEge University [13-TIP-092]en_US
dc.description.sponsorshipWe thank all the patients who participated in the study, also we are grateful to Christen Rune Stensvold in Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark, for D. fragilis-positive control DNA. This study was supported by the grant given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant no.: 13-TIP-092).en_US
dc.identifier.doi10.2478/s11686-018-00017-5en_US
dc.identifier.endpage170en_US
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.issue1en_US
dc.identifier.pmid30645736en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage162en_US
dc.identifier.urihttps://doi.org/10.2478/s11686-018-00017-5
dc.identifier.urihttps://hdl.handle.net/11454/29179
dc.identifier.volume64en_US
dc.identifier.wosWOS:000463575500018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer International Publishing Agen_US
dc.relation.ispartofActa Parasitologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDientamoeba fragilisen_US
dc.subjectPrevalenceen_US
dc.subjectClinical symptomsen_US
dc.subjectSociodemographicen_US
dc.subjectRT-PCRen_US
dc.titleInvestigation of Dientamoeba fragilis Prevalence and Evaluation of Sociodemographic and Clinical Features in Patients with Gastrointestinal Symptomsen_US
dc.typeArticleen_US

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