Comparative evaluation of relaxant effects of three prangos species on mouse corpus cavernosum: Chemical characterization and the relaxant mechanisms of action of P. pabularia and (+)-oxypeucedanin

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dc.authoridyetik-anacak, Gunay/0000-0002-7788-1657
dc.authoridDemiroz Akbulut, Tugce/0000-0002-7606-5316
dc.authoridAlbayrak, Gokay/0000-0002-5729-0796
dc.authoridAlan, Elif/0000-0001-9636-4068
dc.authorscopusid8970943500
dc.authorscopusid57329388100
dc.authorscopusid36988052000
dc.authorscopusid57197819294
dc.authorscopusid57203009049
dc.authorscopusid14032536900
dc.authorscopusid23767071300
dc.authorwosidyetik-anacak, Gunay/A-1418-2018
dc.contributor.authorSevin, Gulnur
dc.contributor.authorAlan, Elif
dc.contributor.authorDemir, Serdar
dc.contributor.authorAlbayrak, Gokay
dc.contributor.authorDemiroz, Tugce
dc.contributor.authorYetik-Anacak, Gunay
dc.contributor.authorBaykan, Sura
dc.date.accessioned2023-01-12T19:55:18Z
dc.date.available2023-01-12T19:55:18Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractEthnopharmacological relevance: Erectile dysfunction (ED) is the most common form of sexual dysfunction which has been the topic of great interest through the history by all cultures. It is now among the most treated health problems in men of all ages that develop under the influence of lifestyle factors and some diseases. Plants are extensively used to cure sexual dysfunction for centuries. Roots of Prangos sp. have been used to improve sexual performance in Anatolian traditional medicine and are rich of coumarin, furanocoumarin and their derivatives. Scientific research is necessary to support and validate the ethno-traditional uses of these plants. Aim of the study: The aim of this study is to investigate the effects of the root extracts of P. pabularia, P. uechtritzii and P. heyniae on erectile function and to isolate and identify the chemical compounds of the most active extract and reveal possible pharmacological mechanism of the major compound of the extract with the strongest relaxant effect in mouse corpus cavernosum (MCC). Materials and methods: The roots of plants were extracted with chloroform, n-hexane and methanol. The compounds were isolated from the extract by column chromatography and structures were identified by NMR and MS. The relaxant effects of extracts (10(-7)-10(-4) g/mL), (+)-oxypeucedanin (10(-7) -10(-4) M) and Na 2 5 (10(-7) -3 x 10(-3) M) were tested in MCC strips by DMT myograph. To investigate the mechanism, the synthesis inhibitors of aminooxyacetic acid (AOAA, 10(-2) M) and nitro-L-arginine methyl ester (L-NAME, 10(-4) M) were used, respectively. H2S formation was evaluated basal and L-cysteine (L-cyst)-stimulated conditions by H2S microsensor. Results: All extracts relaxed MCC in a concentration dependent manner. The maximum relaxing effects were achieved with chloroform extracts. Chloroform extract of P. pabularia (Pp-CE) was more potent than the others. Pp-CE-induced relaxations were significantly decreased by AOAA and L-NAME. (+)-Oxypeucedanin, the major compound of Pp-CE, induced relaxant responses and this effect was inhibited by AOAA, but not L-NAME. The relaxation of (+)-oxypeucedanin was found to be similar in view of E-m(ax) to positive control H2S donor Na2S(+)-Oxypeucedanin increased L-cyst-stimulated H2S formation. Augmentation of H2S synthesis with (+)-oxypeucedanin was inhibited by AOAA. Conclusions: Pp-CE has the strongest effect on relaxation of MCC and this result supports the traditional aphrodisiac use of P. pabularia root extract in Anatolia. The pharmacological mechanisms of Pp-CE to relax MCC involve NO and H2S formation. (+)-Oxypeucedanin could be responsible for the H2S-mediated relaxations of Pp-CE in MCC.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [117-S-116]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Grant number: 117-S-116) .en_US
dc.identifier.doi10.1016/j.jep.2021.114823
dc.identifier.issn0378-8741
dc.identifier.issn1872-7573
dc.identifier.pmid34775035en_US
dc.identifier.scopus2-s2.0-85118898819en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jep.2021.114823
dc.identifier.urihttps://hdl.handle.net/11454/76657
dc.identifier.volume284en_US
dc.identifier.wosWOS:000722317500007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApiaceaeen_US
dc.subjectCoumarinsen_US
dc.subjectAphrodisiacen_US
dc.subjectCorpus cavernosumen_US
dc.subjectHydrogen sulfideen_US
dc.subjectHydrogen-Sulfideen_US
dc.subjectAntioxidant Activitiesen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectErectile Dysfunctionen_US
dc.subjectMedicinal-Plantsen_US
dc.subjectEssential Oilen_US
dc.subjectNitric-Oxideen_US
dc.subjectCoumarinsen_US
dc.subjectConstituentsen_US
dc.subjectApiaceaeen_US
dc.titleComparative evaluation of relaxant effects of three prangos species on mouse corpus cavernosum: Chemical characterization and the relaxant mechanisms of action of P. pabularia and (+)-oxypeucedaninen_US
dc.typeArticleen_US

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