Cryptosporidiosis outbreak on a dairy farm: Detection of Cryptosporidium parvum as a causative agent in the water source

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dc.authoridkarakavuk, muhammet/0000-0002-2468-5564
dc.authoridDoskaya, Mert/0000-0001-6868-008X
dc.authoridCan, Hüseyin/0000-0001-9633-9786
dc.authoridKoseoglu, Ahmet Efe/0000-0002-3505-4397
dc.authorscopusid56495590200
dc.authorscopusid23003351400
dc.authorscopusid23767084500
dc.authorscopusid57330182000
dc.authorscopusid57212213434
dc.authorscopusid25825299300
dc.authorscopusid57219922088
dc.authorwosidkarakavuk, muhammet/ABC-8038-2020
dc.authorwosidDoskaya, Mert/W-4682-2017
dc.authorwosidDegirmenci, Aysu/DET-8805-2022
dc.authorwosidCan, Hüseyin/AAG-3152-2020
dc.authorwosidErkunt Alak, Sedef/AAG-9556-2020
dc.contributor.authorKarakavuk, M.
dc.contributor.authorCan, H.
dc.contributor.authorDoskaya, M.
dc.contributor.authorKarakavuk, T.
dc.contributor.authorErkunt-Alak, S.
dc.contributor.authorKoseoglu, A. E.
dc.contributor.authorGul, A.
dc.date.accessioned2023-01-12T20:12:15Z
dc.date.available2023-01-12T20:12:15Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractDiarrhea caused by parasitic agents is common in neonatal calves and leads to significant economic losses in cattle farms worldwide. Cryptosporidium spp. is one of the most frequently detected parasitic agents causing diarrhea in neonatal calves. The aim of this study was to investigate the presence of Cryptosporidium spp. on a dairy farm which a has major diarrhea problem. Samples were collected from calves, cows, drinking bowls, and two different artesian water sources, as well as from the environment. All fecal samples were investigated using Kinyoun acid-fast stained slides and real-time PCR targeting the Cryptosporidium spp. COWP gene. In addition, species identification was performed by nested PCR targeting the Cryptosporidium spp. COWP gene and sequencing. Cryptosporidium spp. was detected in 11 calves (30.55%; 11/36) by real-time PCR and the cows were negative. Among real-time PCR positive samples, only five were also found positive by microscopy. Moreover, Cryptosporidium spp. was found in one of the two artesian water sources and five environmental samples by real-time PCR. Among these positive samples, eight were sequenced. According to the RFLP pattern, BLAST and, phylogenetic analyses, all sequenced samples were Cryptosporidium parvum. These findings show the importance of C. parvum as a cause of calf diarrhea on dairy farms.en_US
dc.description.sponsorshipEge University Research Fund [TGA 020-20850]en_US
dc.description.sponsorshipAll experiments were performed under the instruc-tions and approval of the Institutional Animal Care and Use Committee (IACUC) of Ege University for animal ethical norms (Permit number: 2019-047) . During the study, permission was obtained from the farm adminis-trative manager for collecting stool samples from calves and cows. This study was financially supported by Ege University Research Fund (Project No: TGA 020-20850) to Muhammet Karakavuk.en_US
dc.identifier.doi10.24425/pjvs.2021.137669
dc.identifier.endpage333en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue3en_US
dc.identifier.pmid34730310en_US
dc.identifier.scopus2-s2.0-85118833661en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage323en_US
dc.identifier.urihttps://doi.org/10.24425/pjvs.2021.137669
dc.identifier.urihttps://hdl.handle.net/11454/78273
dc.identifier.volume24en_US
dc.identifier.wosWOS:000704586300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztynen_US
dc.relation.ispartofPolish Journal of Veterinary Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectneonatal calvesen_US
dc.subjectCryptosporidium parvumen_US
dc.subjectdiarrheaen_US
dc.subjectReal-time PCRen_US
dc.subjectcalf loseen_US
dc.subjectReal-Time Pcren_US
dc.subjectPrevalenceen_US
dc.subjectInfectionen_US
dc.subjectCalvesen_US
dc.subjectGiardiaen_US
dc.subjectDiarrheaen_US
dc.subjectCattleen_US
dc.subjectIdentificationen_US
dc.subjectAssociationen_US
dc.subjectSpecimensen_US
dc.titleCryptosporidiosis outbreak on a dairy farm: Detection of Cryptosporidium parvum as a causative agent in the water sourceen_US
dc.typeArticleen_US

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