Silencing acpP gene via antisense oligonucleotide-niosome complex in clinical Pseudomonas aeruginosa isolates

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dc.authoridERAÇ, BAYRI/0000-0002-6343-2519
dc.authoridKOCAGOZ, TANIL/0000-0001-7211-2026
dc.authorscopusid57196047480
dc.authorscopusid26026210500
dc.authorscopusid6507545730
dc.authorscopusid16202622800
dc.authorscopusid57206183660
dc.authorscopusid8905840200
dc.authorscopusid55917255700
dc.authorwosidtekintas, yamac/AAL-4490-2021
dc.authorwosidERAÇ, BAYRI/AAG-7017-2019
dc.contributor.authorTekintas, Yamac
dc.contributor.authorDemir-Dora, Devrim
dc.contributor.authorErac, Bayri
dc.contributor.authorErac, Yasemin
dc.contributor.authorYilmaz, Ozlem
dc.contributor.authorAydemir, Sabire Sohret
dc.contributor.authorKocagoz, Zuhtu Tanil
dc.date.accessioned2023-01-12T19:56:20Z
dc.date.available2023-01-12T19:56:20Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractPseudomonas aeruginosa, an opportunistic Gram-negative pathogen, is one of the major causes of nosocomial infections. In addition to its physiological adaptation capacity, it can develop resistance to disinfectants and antibiotics through various mechanisms. Recently, new eradication methods are gaining attention. Therefore, in this study, an LNA-2'-O-methyl hybrid antisense oligonucleotide targeting the acyl carrier protein P (acpP) gene was introduced into P. aeruginosa isolates. The design was determined through sequence analysis and prediction of the secondary structure of mRNA by software. Niosomes were used for enhancing cellular uptake. The control of the binding and transfection ability of the sequence was determined fluorometrically by labeling with 6-Fam. The effects were determined with broth microdilution method and qPCR studies. Eight different formulations were prepared. Among these, one formulation has shown to have ASO complexation ability whose composition was 312 mu l Span 80 + 69.5 mg Cholesterol+ 36.4 mg CTAB+1 ml Chloroform and 5 ml dH(2)O. Thus this formulation was determined as the delivery system for the next stages. Significant gene inhibition was detected at the six isolates. Results of this study suggested that niosomes can be used as a delivery system for cellular uptake of ASO and could eliminate bacterial growth. (C) 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [215S824]en_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey by Grant No. 215S824 financially supported this study.en_US
dc.identifier.doi10.1016/j.resmic.2021.103834
dc.identifier.issn0923-2508
dc.identifier.issn1769-7123
dc.identifier.issue4-5en_US
dc.identifier.pmid33894336en_US
dc.identifier.scopus2-s2.0-85109164967en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.resmic.2021.103834
dc.identifier.urihttps://hdl.handle.net/11454/76836
dc.identifier.volume172en_US
dc.identifier.wosWOS:000674401300006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofResearch in Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectMDRen_US
dc.subjectAntisenseen_US
dc.subjectLNAen_US
dc.subject2'-O-Methylen_US
dc.subjectNiosomeen_US
dc.subjectAntibiotic-Resistanceen_US
dc.subjectIn-Vitroen_US
dc.subjectPeptideen_US
dc.subjectInhibitionen_US
dc.subjectGrowthen_US
dc.subjectExpressionen_US
dc.subjectDeliveryen_US
dc.titleSilencing acpP gene via antisense oligonucleotide-niosome complex in clinical Pseudomonas aeruginosa isolatesen_US
dc.typeArticleen_US

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