Effects of disinfectants and ciprofloxacin on quorum sensing genes and biofilm of clinical Pseudomonas aeruginosa isolates

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dc.contributor.authorUzunbayir-Akel, Nilufer
dc.contributor.authorTekintas, Yamac
dc.contributor.authorYilmaz, Fethiye Ferda
dc.contributor.authorOzturk, Ismail
dc.contributor.authorOkeer, Mustafa
dc.contributor.authorAydemir, Sabire Sohret
dc.contributor.authorHosgor-Limoncu, Mine
dc.date.accessioned2021-05-03T20:36:45Z
dc.date.available2021-05-03T20:36:45Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Pseudomonas aeruginosa is one of the most virulent bacteria and quorum sensing (QS) genes have an importance on virulence factors such as biofilm that provide resistance against disinfectants and antibiotics. Objective: This study aimed to determine the minimum inhibitory concentrations of the disinfectants, to investigate the effects of disinfectants and ciprofloxacin on biofilm production mature biofilm of clinical P. aeruginosa isolates, and it was aimed to investigate the effects of the agents on the expression levels of several QS-related genes in the isolates. Methods: Minimum inhibitory concentration (MIC) levels of polyhexamethylene biguanide (PHMB), chlorhexidine (CHX), quaternary ammonium compounds (QAC), glutaraldehyde (GLU) and ciprofloxacin (CIP) against clinical P. aeruginosa isolates were evaluated by microdilution method. Effects of the agents on the biofilm producing capacities of clonally unrelated nine strains were investigated by spectrophotometric method. Alterations in the expression of QS-related genes (lasI, lasR,rhlI and rhlR) were investigated by qPCR in three isolates that were CIP-susceptible and strong biofilm producer. Results: According to microdilution method results, three isolates were found as resistant, one isolate was found as intermediate susceptible and five isolates were found as susceptible to CIP, and CHX (7.81-31.25 pz/mL) had the lowest MIC against P. aeruginosa. CHX inhibited biofilm production levels of eight of nine isolates, and GLU and CIP inhibited six of nine isolates in the presence of agents at MIC levels. GLU inhibited the mature biofilm levels of three of nine isolates at MIC and MIC/4 levels and four of nine isolates at MIC/2 levels. Expression levels of QS-related genes were reduced or induced in the presence of different disinfectants. Conclusions: More efforts are required to decrease the risk of ineffective and low-dose application of disinfectants and antimicrobials against bacteria. Targeting of QS-related genes may be a reasonable strategy for the inhibition of virulence factors in P. aeruginosa. (C) 2020 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences.en_US
dc.description.sponsorshipEge University Scientific Research Projects CommissionEge University [14/ECZ/041]en_US
dc.description.sponsorshipThis study was supported by Ege University Scientific Research Projects Commission with project number 14/ECZ/041.en_US
dc.identifier.doi10.1016/j.jiph.2020.10.002en_US
dc.identifier.endpage1938en_US
dc.identifier.issn1876-0341
dc.identifier.issn1876-035X
dc.identifier.issue12en_US
dc.identifier.pmid33139237en_US
dc.identifier.startpage1932en_US
dc.identifier.urihttps://doi.org/10.1016/j.jiph.2020.10.002
dc.identifier.urihttps://hdl.handle.net/11454/70188
dc.identifier.volume13en_US
dc.identifier.wosWOS:000597948100024en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Londonen_US
dc.relation.ispartofJournal of Infection and Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectDisinfectanten_US
dc.subjectCiprofloxacinen_US
dc.subjectBiofilmen_US
dc.subjectQuorum sensingen_US
dc.titleEffects of disinfectants and ciprofloxacin on quorum sensing genes and biofilm of clinical Pseudomonas aeruginosa isolatesen_US
dc.typeArticleen_US

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