Investigation of iso-propylchaetominine anticancer activity on apoptosis, cell cycle and Wnt signaling pathway in different cancer models

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dc.contributor.authorKaramad, Vahidreza
dc.contributor.authorSogutlu, Fatma
dc.contributor.authorOzkaya, Ferhat Can
dc.contributor.authorShademan, Behrouz
dc.contributor.authorEbrahim, Weaam
dc.contributor.authorEl-Neketi, Mona
dc.contributor.authorAvci, Cigir Biray
dc.date.accessioned2024-08-31T07:50:43Z
dc.date.available2024-08-31T07:50:43Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractDysregulation of the Wnt signaling pathway contributes to the development of many cancer types. Natural compounds produced with biotechnological systems have been the focus of research for being a new drug candidate both with unlimited resources and cost-effective production. In this study, it was aimed to reveal the effects of isopropylchaetominine on cytotoxic, cytostatic, apoptotic and Wnt signaling pathways in brain, pancreatic and prostate cancer. The IC50 values of isopropylchaetominine in U-87 MG, PANC1, PC3 and LNCaP cells were calculated as 91.94 mu M, 41.68 mu M, 54.54 mu M and 7.86 mu M in 72nd h, respectively. The metabolite arrests the cell cycle in G0/G1 phase in each cancer cells. Iso-propylchaetominine induced a 4.3-fold and 1.9-fold increase in apoptosis in PC3 and PANC1 cells, respectively. The toxicity of isopropylchaetominine in healthy fibroblast cells was assessed using the annexin V method, and no significant apoptotic activity was observed between the groups treated with the active substance and untreated. In U-87 MG, PANC1, PC3, and LNCaP cells under treatment with iso-propylchaetominin, the expression levels of DKK3, TLE1, AES, DKK1, FRZB, DAB2, AXIN1/2, PPARD, SFRP4, APC and SOX17 tumor suppressor genes increased significantly. Decreases in expression of Wnt1, Wnt2, Wnt3, Wnt4, Wnt5, Wnt6, Wnt10, Wnt11, FRZ2, FRZ3, FRZ7, TCF7L1, BCL9, PYGO, CCND2, c-MYC, WISP1 and CTNNB1 oncogenic genes were detected. All these result shows that isopropylchaetominine can present promising new treatment strategy in different cancer types.en_US
dc.identifier.doi10.1016/j.fitote.2023.105789
dc.identifier.issn0367-326X
dc.identifier.issn1873-6971
dc.identifier.pmid38158162en_US
dc.identifier.scopus2-s2.0-85181060330en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.fitote.2023.105789
dc.identifier.urihttps://hdl.handle.net/11454/105328
dc.identifier.volume173en_US
dc.identifier.wosWOS:001165807500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofFitoterapiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectWnt Signaling Pathwayen_US
dc.subjectIso-Propylchaetomininen_US
dc.subjectQuinazolinoneen_US
dc.subjectAspergillus Carneusen_US
dc.titleInvestigation of iso-propylchaetominine anticancer activity on apoptosis, cell cycle and Wnt signaling pathway in different cancer modelsen_US
dc.typeArticleen_US

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