Analysis of dysregulated long non-coding RNA expressions in glioblastoma cells

dc.contributor.authorBalci, Tugce
dc.contributor.authorSusluer, Sunde Yilmaz
dc.contributor.authorKayabasi, Cagla
dc.contributor.authorYelken, Besra Ozmen
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorGunduz, Cumhur
dc.date.accessioned2019-10-27T11:26:29Z
dc.date.available2019-10-27T11:26:29Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractLong non coding RNAs (IcRNAs) are associated with various biological roles such as embryogenesis, stem cell biology, cellular development and present specific tissue expression profiles. Aberrant expression of IncRNAs are thought to play a critical role in the progression and development of various cancer types, including gliomas. Glioblastomas (GBM) are common and malignant primary brain tumours. Brain cancer stem cells (BCSC) are isolated from both low and high-grade tumours in adults and children, by cell fraction which express neuronal stem cell surface marker CD133. The purpose of this study was to investigate the expression profiles of IncRNAs in brain tumour cells and determine its potential biological function. For this purpose, U118MG-U87MG; GBM stem cell series were used. Human parental brain cancer cells were included as the control group; the expressions of disease related human IncRNA profiles were studied by LightCycler 480 real-time PCR. Expression profiles of 83 lncRNA genes were analyzed for a significant dysregulation, compared to the control cells. Among IncRNAs, 51 IncRNA genes down-regulated, while 8 IncRNA genes were up-regulated. PCAT-1 (2.36), MEG3 (5.34), HOTAIR (-2.48) lncRNAs showed low expression in glioblastoma compared to the human (parental) brain cancer stem cells, indicating their role as tumour suppressor genes on gliomas. As a result, significant changes for anti-cancer gene expressions were detected with disease-related human IncRNA array plates. Identification of novel target genes may lead to promising developments in human brain cancer treatment. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipResearch Foundation of Ege UniversityEge University [2.101.2014.0014]en_US
dc.description.sponsorshipThis study was supported by the Research Foundation of Ege University (Grant Number 2.101.2014.0014).en_US
dc.identifier.doi10.1016/j.gene.2016.06.024en_US
dc.identifier.endpage122en_US
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.issue1en_US
dc.identifier.pmid27306825en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage120en_US
dc.identifier.urihttps://doi.org/10.1016/j.gene.2016.06.024
dc.identifier.urihttps://hdl.handle.net/11454/33499
dc.identifier.volume590en_US
dc.identifier.wosWOS:000381539800015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLong non coding RNAsen_US
dc.subjectHuman parental brain cancer stem cellen_US
dc.subjectGlioblastomaen_US
dc.titleAnalysis of dysregulated long non-coding RNA expressions in glioblastoma cellsen_US
dc.typeArticleen_US

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