Comparison of two methods for the detection of antituberculous drug susceptibility in mycobacterium tuberculosis complex isolates: Sensititre MycoTB MIC plate and the indirect agar proportion methods [Mycobacterium tuberculosis kompleks İzolatlarının antitüberküloz İlaç duyarlılığını saptamada İki yöntemin karşılaştırılması: Sensititre MycoTB MIC plak ve İndirekt agar proporsiyon Yöntemleri]

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dc.contributor.authorVarici Balci F.K.
dc.contributor.authorÇavuşoğlu C.
dc.date.accessioned2021-05-03T20:48:02Z
dc.date.available2021-05-03T20:48:02Z
dc.date.issued2018
dc.description.abstractTuberculosis is still one of the most important public health problems worldwide. Due to the increase of multidrug-resistant (MDR) tuberculosis and extensively drug-resistant (XDR) tuberculosis cases, fast, practical and standardized methods need to be developed for the determination of drug resistance in Mycobacterium tuberculosis complex. The aim of this study was to compare the Sensititre MycoTB plate method with the gold standard agar proportion method (APM); in order to determine if the Sensititre MycoTB plate method is effective enough to be used instead of the APM. A total of 100 M.tuberculosis complex isolates, 36 with different resistance patterns and 64 sensitive to all first-line agents stored in culture collection of Ege University Faculty of Medicine Microbiology Department Laboratory, were used in the study. The susceptibility of rifampin, isoniazid, ethambutol, streptomycin, ofloxacin, moxifloxacin, amikacin, rifabutin, para-aminosalicylic acid, ethionamide, cycloserine and kanamycin for all the isolates were determined by APM and Sensititre MycoTB plate methods. APM was performed as defined by CLSI, Sensititre MycoTB plate method was performed as instructed by the manufacturer. Results were assessed in 95% confidence interval and sensitivity, specificity and categorical agreement values were determined. Results were obtained in 14 days using the Sensititre MycoTB plate method. Sensitivity and the specificity of the Sensititre MycoTB was between 86-100% and 95-100% respectively, while the categorical agreement between the two methods were 95-100% for the drugs tested. The sensitivities of the drugs were 100% except ethambutol and ethionamide. The specificity values for both of the drugs and categorical agreement were over 95%. All the drugs were in the 95% confidence interval (p< 0.001) and the greatest difference between the lower and upper levels of sensitivity and specificity confidence limits was found in ethambutol and ethionamide. MDR was detected in 16 isolates with the APM and in 20 isolates with the Sensititre MycoTB plate method. None of the isolates had XDR [in addition to MDR resistance against any fluoroquinolones and at Least one of the second-line parenteral drugs (amikacin, kanamycin or capreomycin)]. As a result, owing to the short Incubation period (14 days), high sensitivity, specificity and categorical agreement values, and the possibility for evaluating both first- and second-line agents and the quantitative values of drugs; the Sensititre MycoTB method was determined as an effective method that can be used especially in laboratories where the rate of MDR-TB isolates are high. © 2018 Ankara Microbiology Society. All rights reserved.en_US
dc.identifier.doi10.5578/mb.66683en_US
dc.identifier.endpage134en_US
dc.identifier.issn0374-9096
dc.identifier.issue2en_US
dc.identifier.pmid29933730en_US
dc.identifier.scopus2-s2.0-85054329350en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage123en_US
dc.identifier.urihttps://doi.org/10.5578/mb.66683
dc.identifier.urihttps://hdl.handle.net/11454/70714
dc.identifier.volume52en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Societyen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgar proportion methoden_US
dc.subjectDrug susceptibility testingen_US
dc.subjectMycobacterium tuberculosis complexen_US
dc.subjectSensititre MycoTB plateen_US
dc.titleComparison of two methods for the detection of antituberculous drug susceptibility in mycobacterium tuberculosis complex isolates: Sensititre MycoTB MIC plate and the indirect agar proportion methods [Mycobacterium tuberculosis kompleks İzolatlarının antitüberküloz İlaç duyarlılığını saptamada İki yöntemin karşılaştırılması: Sensititre MycoTB MIC plak ve İndirekt agar proporsiyon Yöntemleri]en_US
dc.typeArticleen_US

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