20 diffüz malign mezotelyoma olgusunun immunohisto-kimyasal analizi
Küçük Resim Yok
Tarih
1998
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Plevranın primer tümörü olan malign mezotelyomaların gerek tanısında gerekse metastatik lezyonlardan, özellikle adenokarsinomdan ayrımında zorluklar yaşanmaktadır. Bu zorlukları ortadan kaldırmada immunohistokimyasal boyama yöntemlerinin kullanılabileceği belirtilmektedir. Çok merkezli olarak planlanan bu çalışmada 20 plevral malign mezotelyoma ve 18 öpere akciğer adenokarsinomu olgusunun parafine gömülü bloklarından yapılan kesitler, immunohistokimyasal yöntem ile vimentin, yüksek molekül ağırlıklı sitokeratin (HMCK), S-100, karsinoembriyonik antijen (CE A), epitelyal membran antijen (EMA), lökosit membran antijenlerinden CD-15 (Leu-M1), HMFG-2, Ber EP4 immunoreaktivitesi yönünden incelendi. Malign mezotelyomada vimentin veya HMCK pozitifliği % 87.5, vimentin ve/veya HMCK pozitifken CD-15 (Leu-M1) negatifliği % 89 oranında saptanırken adenokarsinomlarda vimentin ve HMCK negatifliğiyle birlikte EMA pozitifliği % 94 olarak saptandı. Çalışmanın sonucunda klinik, radyolojik bulgular ve öykü ile birlikte spesifik immunohistokimyasal boyama yöntemlerinin malign mezotelyoma tanı ve ayırıcı tanısında yararlı olabileceği düşünüldü. .
There are difficulties in the diagnosis of pleural mesothelioma (PM), and in the differential diagnosis of this disease from metastatic pleural diseases-especially adenocarcinoma-. It has been reported that immunohistochemical staining methods can be utilized in solving these difficulties. This study planned as a multi-center trial, the histological specimens of 20 cases of malignant PM and 18 of operated pulmonary adenocarcinoma were analyzed by the immunohistochemical staining methods for vimeniin, high molecular weight cytokeratin (HMCK), S-100, carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), one of the leucocyte membrane antigens CD-15 (Leu-M1), human milk fat globulin (HMFG-2) and Ber-EP4. We determined vimentin or HMCK positivity in 87.5 %, vimentin and/or HMCK positivity accompanied by Leu-Mi negativity in 89 % of the patients with PM. In cases of adenocarcinoma vimentin and HMCK negativity was accompanied by EMA positivity< in 94 %. We concluded that specific immunohistochemical staining methods can be usefull in the diagnosis and differential diagnosis of PM in addition to clinical and radiological findings and history.
There are difficulties in the diagnosis of pleural mesothelioma (PM), and in the differential diagnosis of this disease from metastatic pleural diseases-especially adenocarcinoma-. It has been reported that immunohistochemical staining methods can be utilized in solving these difficulties. This study planned as a multi-center trial, the histological specimens of 20 cases of malignant PM and 18 of operated pulmonary adenocarcinoma were analyzed by the immunohistochemical staining methods for vimeniin, high molecular weight cytokeratin (HMCK), S-100, carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), one of the leucocyte membrane antigens CD-15 (Leu-M1), human milk fat globulin (HMFG-2) and Ber-EP4. We determined vimentin or HMCK positivity in 87.5 %, vimentin and/or HMCK positivity accompanied by Leu-Mi negativity in 89 % of the patients with PM. In cases of adenocarcinoma vimentin and HMCK negativity was accompanied by EMA positivity< in 94 %. We concluded that specific immunohistochemical staining methods can be usefull in the diagnosis and differential diagnosis of PM in addition to clinical and radiological findings and history.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Ege Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
3-4
