Fine structural demonstration of anionic sites on streptococcal and staphylococcal envelopes by cationic dyes

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dc.contributor.authorMater Y.
dc.contributor.authorKaraçali S.
dc.date.accessioned2019-10-27T00:25:05Z
dc.date.available2019-10-27T00:25:05Z
dc.date.issued2001
dc.departmentEge Üniversitesien_US
dc.description.abstractAnionic sites on bacterial envelopes of Streptococcus ß-haemolyticus and Staphylococcus aureus ATCC 6538 P were investigated on materials fixed with Karnovsky fixative containing the cationic dyes ruthenium red (RR) or alcian blue (AB). Electron dense granules and short fibrils were found to be irregularly distributed around the cells on the glycocalyces of both genera. An electron dense stained reaction with RR or AB on the cell membrane and glycocalyx indicates the presence of rich anionic sites on these structures. Electron microscopic observations demonstrate that anionic sites in the glycocalyx are responsible for the adherence phenomenon during the formation of microcolonies. For structural improvement of bacterial envelopes, the RR procedure was more effective than the AB procedure.en_US
dc.identifier.endpage295en_US
dc.identifier.issn1300-0144
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage291en_US
dc.identifier.urihttps://hdl.handle.net/11454/23191
dc.identifier.volume31en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlcian Blueen_US
dc.subjectAnionic sitesen_US
dc.subjectGlycocalyxen_US
dc.subjectRuthenium Reden_US
dc.subjectStaphylococcus aureus ATCC 6538 Pen_US
dc.subjectStreptococcus ß-haemolyticusen_US
dc.titleFine structural demonstration of anionic sites on streptococcal and staphylococcal envelopes by cationic dyesen_US
dc.typeArticleen_US

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