Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(epsilon-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations

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dc.authoridMOULAHOUM, Hichem/0000-0003-3934-6415
dc.authoridYagci, Yusuf/0000-0001-6244-6786
dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridCeliker, Tugba/0000-0002-7796-1462
dc.authoridTOK, Kerem/0000-0002-7244-3949
dc.authorscopusid57217737777
dc.authorscopusid57199103659
dc.authorscopusid57188963997
dc.authorscopusid57564391200
dc.authorscopusid57220178117
dc.authorscopusid6602258835
dc.authorscopusid35611300300
dc.authorwosidMOULAHOUM, Hichem/DIC-3335-2022
dc.authorwosidCelik, Emine Guler/AAM-3317-2021
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.authorwosidYagci, Yusuf/AAT-6283-2021
dc.authorwosidTok, Kerem/AAG-6529-2021
dc.contributor.authorCeliker, Tugba
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorGuler Celik, Emine
dc.contributor.authorTok, Kerem
dc.contributor.authorZihnioglu, Figen
dc.contributor.authorCicek, Candan
dc.date.accessioned2023-01-12T19:50:18Z
dc.date.available2023-01-12T19:50:18Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractThe construction of a rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection is described. We report for the first time a novel one-pot synthetic approach for simultaneous photoinduced step-growth polymerization of pyrene (Py) and ring-opening polymerization of epsilon-caprolactone (PCL) to produce a graft fluorescent copolymer PPy-g-PCL that was conjugated to SARS-CoV-2-specific antibodies using EDC/NHS chemistry. The synthesis steps and conjugation products were fully characterized using standard spectral analysis. Next, the PPy-g-PCL was used for the construction of a dot-blot assay which was calibrated for applications to human nasopharyngeal samples. The analytical features of the proposed sensor showed a detection range of 6.03-8.7 LOG viral copy mL(-1) (Ct Scores: 8-25), the limit of detection (LOD), and quantification (LOQ) of 1.84 and 6.16 LOG viral copy mL(-1), respectively. The repeatability and reproducibility of the platform had a coefficient of variation (CV) ranging between 1.2 and 5.9%. The fluorescence-based dot-blot assay was tested with human samples. Significant differences were observed between the fluorescence intensity of the negative and positive samples, with an overall correct response of 93.33%. The assay demonstrated a high correlation with RT-PCR data. This strategy opens new insights into simplified synthesis procedures of the reporter molecules and their high potential sensing and diagnosis applications.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [120C121]; Istanbul Technical University Research Fund [TUB-2021-43195]; Ege University Research Foundation [TOA-2020-21862]; Council of Higher Education of Turkey; Scientific and Technological Research Council of Turkey (TUBITAK)en_US
dc.description.sponsorshipThe authors would like to acknowledge the financial support from The Scientific and Technological Research Council of Turkey (Project Number: 120C121), Istanbul Technical University Research Fund (Project Number: TUB-2021-43195), and Ege University Research Foundation (project number: TOA-2020-21862). One of the authors (T.C.) would like to thank the Council of Higher Education of Turkey for 100/2000 CoHE Doctoral Scholarship and the Scientific and Technological Research Council of Turkey (TUBITAK) for financial support.en_US
dc.identifier.doi10.1007/s00604-022-05244-2
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue5en_US
dc.identifier.pmid35474492en_US
dc.identifier.scopus2-s2.0-85128916703en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s00604-022-05244-2
dc.identifier.urihttps://hdl.handle.net/11454/76068
dc.identifier.volume189en_US
dc.identifier.wosWOS:000788369100003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Wienen_US
dc.relation.ispartofMicrochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPhotopolymerizationen_US
dc.subjectStep-growth polymerizationen_US
dc.subjectRing-opening polymerizationen_US
dc.subjectGraft copolymeren_US
dc.subjectFluorescent biosensoren_US
dc.subjectSARS-CoV-2en_US
dc.subjectLateral Flow Assayen_US
dc.subjectPolymersomesen_US
dc.subjectDiagnosisen_US
dc.titleFluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(epsilon-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizationsen_US
dc.typeArticleen_US

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