Isolation of Chemical Constituents of Centaurea virgata Lam. and Xanthine Oxidase Inhibitory Activity of the Plant Extract and Compounds

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dc.contributor.authorTuezuen, Burcu S.
dc.contributor.authorHajdu, Zsuzsanna
dc.contributor.authorOrban-Gyapai, Orsolya
dc.contributor.authorZomborszki, Zoltan P.
dc.contributor.authorJedlinszki, Nikoletta
dc.contributor.authorForgo, Peter
dc.contributor.authorKivcak, Bijen
dc.contributor.authorHohmann, Judit
dc.date.accessioned2019-10-27T11:21:23Z
dc.date.available2019-10-27T11:21:23Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Centaurea virgata Lam. is a species widely used in the traditional medicine in Turkey for the treatment of diabetes, allergy and gastric ulcers. The rationale of its use in the therapy has not been studied previously, therefore the present work aimed at the chemicalpharmacological evaluation of the plant. Objective: The xanthine oxidase (XO) inhibitory activity of the MeOH extract and its subextracts (n-hexane, CHCl3 and remaining MeOH-H2O) prepared from C. virgata was investigated in vitro. Moderate activity was exerted in case of the CHCl3 extract (98.9 +/- 15.8 mu g/mL), therefore constituents of this extract were analysed. Method: Different purification steps, such as VLC, CPC, PLC and crystallization were used for the isolation, and ESIMS, NMR, LC-MS and authentic standards were applied for identification of the compounds. XO inhibitory and DPPH assays were used for evaluation of the bioactivities. Results: Sesquiterpenes [8 alpha-hydroxysonchucarpolide, 8 alpha-(3,4-dihydroxy-2-methylenebutanoyloxy)-dehydromelitensine, and cnicin], flavones (apigenin, hispidulin, salvigenin, eupatorin, 3'-methyleupatorin), and the flavonol isokaempferide were isolated from the active extract. The XO-inhibitory activity of these compounds was analyzed using allopurinol as a positive control (IC50 7.49 +/- 0.29 +/- M). It was found that sesquiterpenes and flavonoids, containing 7OMe group, are inactive. Conclusion: 7-Hydroxyflavones (apigenin and hispidulin) exerted significant XO inhibitory effect with IC50 values of 0.99 +/- 0.33 +/- M and 4.88 +/- 1.21 +/- M, respectively. Therefore, these compounds are responsible for the XO-inhibitory effect of the extract. The free radical scavenging activity of the isolated flavonoids was determined by DPPH assay, and it was stated that none of the compounds have substantial antioxidant activity, therefore the reduced generation of reactive oxygen species may be the consequence only of XO inhibition.en_US
dc.description.sponsorshipHungarian Scientific Research Fund (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K109846]en_US
dc.description.sponsorshipFinancial support from the Hungarian Scientific Research Fund (OTKA K109846) is gratefully acknowledged.en_US
dc.identifier.doi10.2174/1573406413666161219161946en_US
dc.identifier.endpage502en_US
dc.identifier.issn1573-4064
dc.identifier.issn1875-6638
dc.identifier.issue5en_US
dc.identifier.pmid27991400en_US
dc.identifier.startpage498en_US
dc.identifier.urihttps://doi.org/10.2174/1573406413666161219161946
dc.identifier.urihttps://hdl.handle.net/11454/32911
dc.identifier.volume13en_US
dc.identifier.wosWOS:000405545000010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofMedicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCentaurea virgataen_US
dc.subjectasteraceaeen_US
dc.subjectxanthine oxidase inhibitory activityen_US
dc.subjectflavonoidsen_US
dc.subjectsesquiterpenesen_US
dc.titleIsolation of Chemical Constituents of Centaurea virgata Lam. and Xanthine Oxidase Inhibitory Activity of the Plant Extract and Compoundsen_US
dc.typeArticleen_US

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