Effect of some biocides on glutathione-s-transferase in barley, wheat, lentil and chickpea plants
Küçük Resim Yok
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Glutationun herbisitler ve insektisitlerle konjugasyonu, bitkilerin detoksifikasyon mekanizmalarının en Önemlilerinden biridir. Bu konjugasyon, glutation-s-transferazlar olarak bilinen multifonksiyonel bir enzim ailesi taraf?ndan katalizlenir. Buğday (Triticum aestivum L.cv.Cumhuriyet-75), arpa (Hordeum vulgare L.cv.Kaya), nohut (Cicer arietinum L.cv.Üspanyol) ve mercimeğin (Lens culinaris Medik.cv.Kışlık-kırmızı-51) seÇilen herbisitler; 2,4-D (2,4-dikloro fenoksiasetik asit), linuron (N-(3,4-diklorofenil)-N«- metoksi-N«-metil Üre) ve insektisitler; paratiyon metil (O,O-dimetil O-(4-nitrofenil)fosforotiyoat), malatiyon (S-1,2- bis(etoksibikarbonil)etil O,O-dimetil fosforoditiyoat) ile muamelesi, köklerde ve filizlerdeki glutation-s-transferaz aktivitesinde yükselen bir artışa sebep olduÛu görülmüştür. Enzim aktivitesinde maksimum artış, 1,37 mM paratiyon metille muamele edilmiş buÛday köklerinde kontrolün %485'i olarak gözlenmiştir. Benzer, fakat daha düşük bir artış, bitkilerin kök ve filizleri için, orijine ve pestisit türüne göre farklanarak glutation (GSH) düzeylerinde gözlenmiştir.
The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat (Triticum aestivum L. cv. Cumhuriyet-75), barley (Hordeum vulgare L. cv. Kaya), chickpea (Cicer arietinum L. cv. Ispanyol), and lentil (Lens culinaris Medik. cv. Kışlık-kırmızı-51) with the selective herbicides 2,4-D (2,4-Dichloro phenoxyacetic acid) and linuron (N-(3,4-dichlorophenyl)-N´-methoxy-N´-methyl urea), and insecticides parathion methyl (O,O-dimethyl O-(4-nitrophenyl) phosphorothioate) and malathion (S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethyl phosphorodithioate) caused a progressive increase in glutathione-s-transferase activity in roots and shoots. the maximum increase in enzyme activity was observed in parathion methyl treated wheat roots: 485% of control with a parathion methyl concentration of 1.37 mM. A similar but lower increase i n glutathione (GSH) levels was observed for roots and shoots, differing according to the origin and the type of pesticide.
The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat (Triticum aestivum L. cv. Cumhuriyet-75), barley (Hordeum vulgare L. cv. Kaya), chickpea (Cicer arietinum L. cv. Ispanyol), and lentil (Lens culinaris Medik. cv. Kışlık-kırmızı-51) with the selective herbicides 2,4-D (2,4-Dichloro phenoxyacetic acid) and linuron (N-(3,4-dichlorophenyl)-N´-methoxy-N´-methyl urea), and insecticides parathion methyl (O,O-dimethyl O-(4-nitrophenyl) phosphorothioate) and malathion (S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethyl phosphorodithioate) caused a progressive increase in glutathione-s-transferase activity in roots and shoots. the maximum increase in enzyme activity was observed in parathion methyl treated wheat roots: 485% of control with a parathion methyl concentration of 1.37 mM. A similar but lower increase i n glutathione (GSH) levels was observed for roots and shoots, differing according to the origin and the type of pesticide.
Açıklama
Anahtar Kelimeler
Biyoloji
Kaynak
Turkish Journal of Biology
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
2
