The effect of exogenous melatonin administration on trabecular width, ligament thickness and TGF-beta(1) expression in degenerated intervertebral disk tissue in the rat

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dc.contributor.authorTurgut, M
dc.contributor.authorOktem, G
dc.contributor.authorUslu, S
dc.contributor.authorYurtseven, ME
dc.contributor.authorAktug, H
dc.contributor.authorUysal, A
dc.date.accessioned2019-10-27T19:19:12Z
dc.date.available2019-10-27T19:19:12Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractIntervertebral disk (IVD) degeneration, a complex pathological condition of varying origins, causes low back pain. Degenerative changes in IVD tissue affect the adjacent vertebral structure, resulting in a decreased vertebral trabecular width. It has been suggested that transforming growth factor-beta 1 (TGF-beta(1)) may have a role in the repair of connective tissue, as it occurs in the IVD degeneration process. In this study, we investigated the effects of exogenous melatonin (MEL) administration on vertebral trabecular width, ligament thickness and TGF-beta(1) expression in degenerated IVD tissue. Fifteen adult male Swiss Albino rats were divided randomly into three groups; nonoperated control, operated degeneration, and MEL treatment groups. In the operated degeneration and MEL treatment groups, cuts were made parallel to the end plates in the posterior annulus fibrosus at the fifth and tenth vertebral segments of the tail to induce IVD degeneration. In each group, TGF-beta(1) immunoreactivity and morphometry of vertebral trabecular width and anterior and posterior ligament thickness were evaluated. Histologically, disorganisation and irregularity of collagen fibres was seen in the degenerated (operated) IVD. Increased TGF-beta(1) expression in multinuclear chondrocytes was also observed as was decreased vertebral trabecular width. Importantly, the reduction of trabecular width observed in the operated degenerated group was reversed after MEL administration (p<0.0001). Similarly, TGF-beta(1) expression in multinuclear chondrocytes was dramatically increased after exogenous MEL application. Thus, there was a regression in histopathological changes after MEL treatment, with disk appearances similar to those of the control group. Based on our findings. we suggest that MEL activates the recovery process in the degenerated IVD tissue, possibly by stimulating TGF-beta(1) activity. This is the first report investigating the involvement of the pineal hormone MEL in the repair of rat IVD. (C) 2006 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.jocn.2005.03.037en_US
dc.identifier.endpage363en_US
dc.identifier.issn0967-5868
dc.identifier.issue3en_US
dc.identifier.pmid16546388en_US
dc.identifier.startpage357en_US
dc.identifier.urihttps://doi.org/10.1016/j.jocn.2005.03.037
dc.identifier.urihttps://hdl.handle.net/11454/38707
dc.identifier.volume13en_US
dc.identifier.wosWOS:000236975700012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofJournal of Clinical Neuroscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmelatoninen_US
dc.subjectTGF-beta(1)en_US
dc.subjectdegenerationen_US
dc.subjectintervertebral disken_US
dc.subjectvertebral trabeculaen_US
dc.subjectraten_US
dc.titleThe effect of exogenous melatonin administration on trabecular width, ligament thickness and TGF-beta(1) expression in degenerated intervertebral disk tissue in the raten_US
dc.typeArticleen_US

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