Ultrasensitive covalently-linked Aptasensor for cocaine detection based on electrolytes-induced repulsion/attraction of colloids

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dc.contributor.authorSanli, Serdar
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorCelik, Emine Guler
dc.contributor.authorTimur, Suna
dc.date.accessioned2020-12-01T11:58:39Z
dc.date.available2020-12-01T11:58:39Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractA quick and easy colorimetric sensor based on gold nanoparticles (GNPs) and aptamers for the detection of cocaine was developed. the sensor was named as 'GAPTA' and showed extremely interesting results regarding cocaine detection with a sensitivity to doses of 0.2 nM. the experimental approach consisted of creating a conjugate between GNPs (10 nm size) and aptamers as a sensing base with the addition of an electrolyte (NaCl) that plays the role of aggregation inducer. in the absence of the aptamer, the electrolyte was able to induce aggregation of the GNPs turning the color of the solution from red to blue while the presence of the aptamer is able to hinder the charges attraction and protects the GNPs from aggregating. the optimization of the aptamer and electrolyte concentration was determined to be 118 nM and 55 mM, respectively, and the resultant GAPTA sensor had a detection limit of 0.97 nM. Furthermore, the selectivity of the platform was tested in the presence of different interferents and showed a specific response towards cocaine while interference ranged between 20 and 40%. the applicability of the GAPTA biosensor was tested on synthetic saliva and demonstrated a sensitivity range between 0.2 and 25 nM. These results suggest the potential of the current colorimetric sensor in abuse drugs screening and creates a stable base for new routine platforms for biomedical and toxicology applications. Graphical abstracten_US
dc.description.sponsorshipRepublic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016 K121190]; Aliye Uster Foundationen_US
dc.description.sponsorshipThis work was supported by the Republic of Turkey, Ministry of Development (Project Grant No: 2016 K121190). Part of the work was funded by Aliye Uster Foundation.en_US
dc.identifier.doi10.1007/s10544-020-00507-2en_US
dc.identifier.issn1387-2176
dc.identifier.issn1572-8781
dc.identifier.issue3en_US
dc.identifier.pmid32748213en_US
dc.identifier.scopus2-s2.0-85088899705en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s10544-020-00507-2
dc.identifier.urihttps://hdl.handle.net/11454/62074
dc.identifier.volume22en_US
dc.identifier.wosWOS:000556005000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiomedical Microdevicesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptasensoren_US
dc.subjectGold nanoparticlesen_US
dc.subjectAptameren_US
dc.subjectCocaineen_US
dc.subjectDrug detectionen_US
dc.titleUltrasensitive covalently-linked Aptasensor for cocaine detection based on electrolytes-induced repulsion/attraction of colloidsen_US
dc.typeArticleen_US

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