Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Study

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dc.contributor.authorOnak, Gunnur
dc.contributor.authorSen, Mustafa
dc.contributor.authorHorzum, Nesrin
dc.contributor.authorErcan, Utku Kursat
dc.contributor.authorYarali, Ziysan Buse
dc.contributor.authorGaripcan, Bora
dc.contributor.authorKaraman, Ozan
dc.date.accessioned2019-10-27T09:59:31Z
dc.date.available2019-10-27T09:59:31Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractOptimization of nanofiber (NF) surface properties is critical to achieve an adequate cellular response. Here, the impact of conjugation of biomimetic aspartic acid (ASP) and glutamic acid (GLU) templated peptides with poly(lactic-co-glycolic acid) (PLGA) electrospun NF on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) was evaluated. Cold atmospheric plasma (CAP) was used to functionalize the NF surface and thus to mediate the conjugation. The influence of the CAP treatment following with peptide conjugation to the NF surface was assessed using water contact angle measurements, Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS). The effect of CAP treatment on morphology of NF was also checked using Scanning Electron Microscopy (SEM). Both the hydrophilicity of NF and the number of the carboxyl (-COOH) groups on the surface increased with respect to CAP treatment. Results demonstrated that CAP treatment significantly enhanced peptide conjugation on the surface of NF. Osteogenic differentiation results indicated that conjugating of biomimetic ASP templated peptides sharply increased alkaline phosphatase (ALP) activity, calcium content, and expression of key osteogenic markers of collagen type I (Col-I), osteocalcin (OC), and osteopontin (OP) compared to GLU conjugated (GLU-pNF) and CAP treated NF (pNF). It was further depicted that ASP sequences are the major fragments that influence the mineralization and osteogenic differentiation in non-collagenous proteins of bone extracellular matrix.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [117S429]; BAP (Scientific Research Projects Fund of Izmir Katip Celebi University) [2016-ONP-MUMF-0022]en_US
dc.description.sponsorshipAuthors acknowledge funding from TUBITAK (The Scientific and Technological Research Council of Turkey) through the Research Project 117S429 and BAP (Scientific Research Projects Fund of Izmir Katip Celebi University) through the Research Projects of 2016-ONP-MUMF-0022. Finally, authors would like to acknowledge Bonegraft Biomaterials Co. (Izmir, Turkey) for providing DNA quantification kit, respectively.en_US
dc.identifier.doi10.1038/s41598-018-36109-5en_US
dc.identifier.issn2045-2322
dc.identifier.pmid30514892en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-018-36109-5
dc.identifier.urihttps://hdl.handle.net/11454/29636
dc.identifier.volume8en_US
dc.identifier.wosWOS:000452084600028en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Studyen_US
dc.typeArticleen_US

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