Impedimetric Detection of microRNA at Graphene Oxide Modified Sensors

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dc.contributor.authorCongur G.
dc.contributor.authorEksin E.
dc.contributor.authorErdem A.
dc.date.accessioned2019-10-26T21:20:48Z
dc.date.available2019-10-26T21:20:48Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractAbstract The graphene oxide (GO) modified pencil graphite electrodes (PGEs) were utilized for electrochemical monitoring of microRNA-34a (miRNA-34a) in this study. The GO concentration was firstly optimized after its modification onto the surface of disposable PGE by passive adsorption. The electrochemical behaviour of GO-PGEs was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) as well as microscopic characterization by using scanning electron microscopy (SEM). The impedimetric detection of hybridization between miRNA-34a target and its complementary DNA probe was recognized under the optimum conditions. The selectivity of the impedimetric genosensor was furtherly studied against to other miRNA sequences; such as; miRNA-15a, miRNA-155 and miRNA-660 in PBS (pH 7.40) or fetal bovine serum (FBS): PBS (1:1) diluted solution. © 2015 Elsevier Ltd.en_US
dc.identifier.doi10.1016/j.electacta.2015.03.210
dc.identifier.endpage27en_US
dc.identifier.issn0013-4686
dc.identifier.issn0013-4686en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage20en_US
dc.identifier.urihttps://doi.org/10.1016/j.electacta.2015.03.210
dc.identifier.urihttps://hdl.handle.net/11454/16804
dc.identifier.volume172en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofElectrochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectelectrochemical impedance spectroscopyen_US
dc.subjectfetal bovine serumen_US
dc.subjectGraphene oxideen_US
dc.subjectmicroRNAen_US
dc.subjectpencil graphite electrodeen_US
dc.titleImpedimetric Detection of microRNA at Graphene Oxide Modified Sensorsen_US
dc.typeArticleen_US

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