Impedimetric Detection of microRNA at Graphene Oxide Modified Sensors
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Abstract The graphene oxide (GO) modified pencil graphite electrodes (PGEs) were utilized for electrochemical monitoring of microRNA-34a (miRNA-34a) in this study. The GO concentration was firstly optimized after its modification onto the surface of disposable PGE by passive adsorption. The electrochemical behaviour of GO-PGEs was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) as well as microscopic characterization by using scanning electron microscopy (SEM). The impedimetric detection of hybridization between miRNA-34a target and its complementary DNA probe was recognized under the optimum conditions. The selectivity of the impedimetric genosensor was furtherly studied against to other miRNA sequences; such as; miRNA-15a, miRNA-155 and miRNA-660 in PBS (pH 7.40) or fetal bovine serum (FBS): PBS (1:1) diluted solution. © 2015 Elsevier Ltd.
Açıklama
Anahtar Kelimeler
electrochemical impedance spectroscopy, fetal bovine serum, Graphene oxide, microRNA, pencil graphite electrode
Kaynak
Electrochimica Acta
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
172
