A comparative study of the effects of cholesterol and desmosterol on zwitterionic DPPC model membranes

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dc.contributor.authorAltunayar, Cisem
dc.contributor.authorSahin, Ipek
dc.contributor.authorKazanci, Nadide
dc.date.accessioned2019-10-27T22:27:02Z
dc.date.available2019-10-27T22:27:02Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractDesmosterol is a direct biosynthetic precursor of cholesterol in Bloch biochemical pathway of cholesterol biosynthesis and differs with cholesterol only by a double bond in carbon 24. In this study, we aimed to research for the first time comparative effects of cholesterol and its precursor desmosterol on dipalmitoyl phosphatidylcholine (DPPC) multilamellar vesicles (MLVs) by utilizing Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Our DSC studies reveal that with the addition of increasing desmosterol and cholesterol concentrations into pure DPPC MLVs, the pretransition disappears, the main phase transition shifts to lower temperatures and then disappears. While the main phase transition is abolished at 25 mol% concentration of desmosterol, this disappearance of the main phase transition occurs at cholesterol concentration above 30 mol%. Our FTIR studies show that both desmosterol and cholesterol decrease the order in the gel phase, whereas they increase it in the liquid crystalline phase. Importantly, we found that the effect of desmosterol on membrane order is weaker than that of cholesterol in both phases. Moreover, desmosterol and cholesterol increase the dynamics of DPPC membranes in the gel phase, while they decrease it in the liquid crystalline phase. Both sterols also induce a decrease in the wavenumber values of the C=O stretching and PO2- antisymmetric double stretching bands of DPPC both in the gel and liquid crystalline phases, which points out hydrogen bonding in between the hydroxyl group of both sterols and the carbonyl and phosphate groups of DPPC membranes. (C) 2015 Elsevier Ireland Ltd. All rights reserved.en_US
dc.description.sponsorshipEge University Research FundEge University [2011 FEN 046]en_US
dc.description.sponsorshipThis work was supported by Ege University Research Fund 2011 FEN 046. We would like to thank Prof. Dr. Feride Severcan of the Department of Biological Sciences at Middle East Technical University for the generous use of her laboratory.en_US
dc.identifier.doi10.1016/j.chemphyslip.2015.03.006en_US
dc.identifier.endpage45en_US
dc.identifier.issn0009-3084
dc.identifier.issn1873-2941
dc.identifier.pmid25847673en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage37en_US
dc.identifier.urihttps://doi.org/10.1016/j.chemphyslip.2015.03.006
dc.identifier.urihttps://hdl.handle.net/11454/50545
dc.identifier.volume188en_US
dc.identifier.wosWOS:000356743700004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofChemistry and Physics of Lipidsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCholesterolen_US
dc.subjectDesmosterolen_US
dc.subjectDipalmitoyl phosphatidylcholineen_US
dc.subjectMembraneen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectDifferential scanning calorimetryen_US
dc.titleA comparative study of the effects of cholesterol and desmosterol on zwitterionic DPPC model membranesen_US
dc.typeArticleen_US

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