Optimized aptamer-based next generation biosensor for the ultra-sensitive determination of SARS-CoV-2 S1 protein in saliva samples

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dc.authorid0000-0002-4375-8386
dc.authorid0000-0002-3268-8319
dc.authorid0000-0002-8094-8277
dc.contributor.authorErdem, Arzum
dc.contributor.authorSenturk, Huseyin
dc.contributor.authorYildiz, Esma
dc.contributor.authorMaral, Meltem
dc.date.accessioned2025-04-21T08:31:38Z
dc.date.available2025-04-21T08:31:38Z
dc.date.issued2024
dc.departmentEge Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractCOVID-19 is an infectious disease caused by the SARS-CoV-2 virus, which rapidly spread worldwide and resulted in a pandemic. Efficient and sensitive detection techniques have been devised since the onset of the epidemic and continue to be improved at present. Due to the crucial role of the SARS-CoV-2 S1 protein in facilitating the virus's entry into cells, efforts in detection and treatment have primarily centered upon this protein. In this study, a rapid, ultrasensitive, disposable, easy-to-use, cost-effective next generation biosensor based on optimized aptamer (Optimer, OPT) was developed by using a disposable pencil graphite electrode (PGE) and applied for the impedimetric determination of SARS-CoV-2 S1 protein. The S1 protein interacted with the OPT in the solution phase and then immobilized onto the PGE surface. Subsequently, measurements using electrochemical impedance spectroscopy (EIS) were conducted in a solution containing a redox probe of 1 mM [Fe(CN)6]3- /4- . Under optimum conditions, the limit of detection (LOD) for the S1 protein in buffer medium at concentrations ranging from 101 to 106 ag/mL was calculated as 8.80 ag/mL (0.11 aM). The selectivity of the developed biosensor was studied against MERS-CoV-S1 protein (MERS) and Influenza Hemagglutinin antigen (HA). Furthermore, the application of the biosensor in artificial saliva medium is demonstrated. The LOD was also calculated in artificial saliva medium in the concentration range of 101-105 ag/mL and calculated as 2.01 ag/mL (0.025 aM). This medium was also used to assess the selectivity of optimized-aptamer based biosensor.
dc.identifier.citationErdem, A., Senturk, H., Yildiz, E., & Maral, M. (2024). Optimized aptamer-based next generation biosensor for the ultra-sensitive determination of SARS-CoV-2 S1 protein in saliva samples. International Journal of Biological Macromolecules, 281(Pt 1), 136233.
dc.identifier.doi10.1016/j.ijbiomac.2024.136233
dc.identifier.endpage9
dc.identifier.issnhttps://doi.org/10.1016/j.ijbiomac.2024.136233
dc.identifier.issueNov
dc.identifier.pmid39362419
dc.identifier.scopus2-s2.0-85205951088
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.136233
dc.identifier.urihttps://hdl.handle.net/11454/117125
dc.identifier.volume281
dc.identifier.wosWOS:001335278600001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErdem, Arzum
dc.institutionauthorSenturk, Huseyin
dc.institutionauthorYildiz, Esma
dc.institutionauthorMaral, Meltem
dc.institutionauthorid0000-0002-4375-8386
dc.institutionauthorid0000-0002-3268-8319
dc.institutionauthorid0000-0002-8094-8277
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCOVID-19
dc.subjectElectrochemical aptasensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectInfectious disease
dc.subjectOptimer
dc.subjectOptimized aptamer
dc.subjectSARS-CoV-2 S1 protein
dc.titleOptimized aptamer-based next generation biosensor for the ultra-sensitive determination of SARS-CoV-2 S1 protein in saliva samples
dc.typeArticle

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