Detection of phenolic compounds by thick film sensors based on Pseudomonas putida

Basit Öğe Kaydı
dc.contributor.authorTimur, S
dc.contributor.authorPazarlioglu, N
dc.contributor.authorPilloton, R
dc.contributor.authorTelefoncu, A
dc.date.accessioned2019-10-27T18:59:02Z
dc.date.available2019-10-27T18:59:02Z
dc.date.issued2003
dc.departmentEge Üniversitesien_US
dc.description.abstractAmperometric biosensors using bacterial cells were developed for the determination of phenolic compounds and the measurement was based on the respiratory activity of the cells. For this purpose, Pseudomonas putida DSM 50026 which is one of the well-known phenol degrading organisms, was used as a biological component. The cells were grown in the presence of phenol as the sole source of organic carbon. As well as phenol adapted cells, the bacterium which used the glucose as the major carbon source, was also used to obtain another type of biosensor for the comparison of the responses and specificities towards different xenobiotics. The commercial oxygen electrode was used as a transducer to test the sensor responses for both induced and non-induced cells. Our results showed that the adaptation step enable us to obtain biosensor devices with different substrate specificity. Moreover, P. putida was immobilized on the surface of thick film working electrodes made of gold by using gelatin membrane cross-linked with glutaraldehyde. The biosensors were calibrated for different phenolic substances. Furthermore, phenol detection was performed in synthetic wastewater samples. (C) 2003 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.doi10.1016/S0039-9140(03)00237-6en_US
dc.identifier.endpage93en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.issue2en_US
dc.identifier.pmid18969166en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage87en_US
dc.identifier.urihttps://doi.org/10.1016/S0039-9140(03)00237-6
dc.identifier.urihttps://hdl.handle.net/11454/37575
dc.identifier.volume61en_US
dc.identifier.wosWOS:000186127200003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmicrobial biosensoren_US
dc.subjectthick film electrodesen_US
dc.subjectPseudomonas putidaen_US
dc.titleDetection of phenolic compounds by thick film sensors based on Pseudomonas putidaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu