Lipase biosensor for tributyrin and pesticide detection

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dc.contributor.authorKartal, Funda
dc.contributor.authorKilinc, Ali
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T19:38:02Z
dc.date.available2019-10-27T19:38:02Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description7th Workshop on Biosensors and BioAnalytical Mu-Techniques for Environmental and Clinical Analysis -- SEP 10-14, 2006 -- Kusadasi, TURKEYen_US
dc.description.abstractPotentiometric biosensors based on Candida rugosa lipase was described for the detection of organophosphorus pesticide; methyl-parathion and tributyrin. Lipase was immobilized on the glass electrode by means of a gelatin membrane, which is then cross-linked with glutaraldehyde. The principle of the biosensor is based on the measurement of pH variation which was recorded in millivolts due to the enzymatic hydrolysis of tributyrin to butyric acid. For the inhibitor detection, biosensor responses were measured after pesticide treatment, which caused a drop in enzyme activity because of the irreversible inhibition. Reactivation conditions of the reused enzyme electrodes were also investigated by pyridine-2-aldoxime methiodide (2-PAM). The limit of detection for tributyrin was estimated as 93 mu M for lipase sensor within the linear range of 65-455 mu M.en_US
dc.identifier.doi10.1080/03067310701327741
dc.identifier.endpage722en_US
dc.identifier.issn0306-7319
dc.identifier.issn0306-7319en_US
dc.identifier.issue10.Noven_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage715en_US
dc.identifier.urihttps://doi.org/10.1080/03067310701327741
dc.identifier.urihttps://hdl.handle.net/11454/40041
dc.identifier.volume87en_US
dc.identifier.wosWOS:000248051600004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofInternational Journal of Environmental Analytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectbiosensoren_US
dc.subjectorganophosphorus pesticideen_US
dc.subjecttributyrinen_US
dc.titleLipase biosensor for tributyrin and pesticide detectionen_US
dc.typeArticleen_US

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