Lipase biosensor for tributyrin and pesticide detection
Küçük Resim Yok
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Potentiometric biosensors based on Candida rugosa lipase was described for the detection of organophosphorus pesticide; methyl-parathion and tributyrin. Lipase was immobilized on the glass electrode by means of a gelatin membrane, which is then cross-linked with glutaraldehyde. The principle of the biosensor is based on the measurement of pH variation which was recorded in millivolts due to the enzymatic hydrolysis of tributyrin to butyric acid. For the inhibitor detection, biosensor responses were measured after pesticide treatment, which caused a drop in enzyme activity because of the irreversible inhibition. Reactivation conditions of the reused enzyme electrodes were also investigated by pyridine-2-aldoxime methiodide (2-PAM). The limit of detection for tributyrin was estimated as 93 mu M for lipase sensor within the linear range of 65-455 mu M.
Açıklama
7th Workshop on Biosensors and BioAnalytical Mu-Techniques for Environmental and Clinical Analysis -- SEP 10-14, 2006 -- Kusadasi, TURKEY
Anahtar Kelimeler
Candida rugosa lipase, biosensor, organophosphorus pesticide, tributyrin
Kaynak
International Journal of Environmental Analytical Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
87
Sayı
10.Nov
