Construction of Mesenchymal Stem Cell-Derived Artificial Human Urinary Bladder: A Preliminary Study

Basit Öğe Kaydı
dc.contributor.authorTayhan, Seçil Erden
dc.contributor.authorŞendemir, Aylin
dc.contributor.authorMir, Erol
dc.contributor.authorGürhan, İsmet Deliloğlu
dc.date.accessioned2024-08-31T07:31:28Z
dc.date.available2024-08-31T07:31:28Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: The present study aimed to obtain the required cells and select a suitable scaffold material for constructing an artificial bladder using the tissue engineering approach. Materials and methods: The convenience of obtaining human adipose tissue-derived stem cells (hADMSCs) was used in this study. It was attempted to differentiate these cells into smooth muscle cells (SMC), which are present along the wall of the bladder. Urothelial cells were enzymatically isolated from tissue biopsies. Synthetic (poly-lactide co-glycolic acid, PLGA) and natural (chitosan) polymers were used in scaffold fabrication using a tissue engineering approach. Results: In the cellular experiments, urothelial cells couldn’t be cultured in polystyrene culture vessels in vitro and required a support material to maintain viability. Better results were obtained with the feeder layer. The hADMSCs exhibited the expected morphological changes in the serum-rich medium content in the SMC differentiation experiments. Chitosan, biocompatible and biodegradable, was mixed with PLGA as an alternative scaffold combination. Conclusion: This study indicated that hADMSCs-derived smooth muscle cells and biopsy-isolated urothelial cells cultured on hybrid chitosan–PLGA scaffolds with appropriate physical properties could serve as a suitable model for tissue-engineered artificial bladder construction.en_US
dc.identifier.doi10.26453/otjhs.1402217
dc.identifier.endpage72en_US
dc.identifier.issn2459-1467
dc.identifier.issue1en_US
dc.identifier.startpage66en_US
dc.identifier.trdizinid1227061en_US
dc.identifier.urihttps://doi.org/10.26453/otjhs.1402217
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1227061
dc.identifier.urihttps://hdl.handle.net/11454/103455
dc.identifier.volume9en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofOnline Türk Sağlık Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectMesenchymal stem cellen_US
dc.subjecttissue engineeringen_US
dc.subjecturinary bladderen_US
dc.subjecturothelial cellsen_US
dc.titleConstruction of Mesenchymal Stem Cell-Derived Artificial Human Urinary Bladder: A Preliminary Studyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu