Comprehensive Snake Venomics of the Okinawa Habu Pit Viper, Protobothrops flavoviridis, by Complementary Mass Spectrometry-Guided Approaches

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dc.contributor.authorDamm, Maik
dc.contributor.authorHempel, Benjamin-Florian
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorSuessmuth, Roderich D.
dc.date.accessioned2019-10-27T10:03:35Z
dc.date.available2019-10-27T10:03:35Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractThe Asian world is home to a multitude of venomous and dangerous snakes, which are used to induce various medical effects in the preparation of traditional snake tinctures and alcoholics, like the Japanese snake wine, named Habushu. The aim of this work was to perform the first quantitative proteomic analysis of the Protobothrops flavoviridis pit viper venom. Accordingly, the venom was analyzed by complimentary bottom-up and top-down mass spectrometry techniques. The mass spectrometry-based snake venomics approach revealed that more than half of the venom is composed of different phospholipases A2 (PLA(2)). The combination of this approach and an intact mass profiling led to the identification of the three main Habu PLA(2)s. Furthermore, nearly one-third of the total venom consists of snake venom metalloproteinases and disintegrins, and several minor represented toxin families were detected: C-type lectin-like proteins (CTL), cysteine-rich secretory proteins (CRISP), snake venom serine proteases (svSP), l-amino acid oxidases (LAAO), phosphodiesterase (PDE) and 5'-nucleotidase. Finally, the venom of P. flavoviridis contains certain bradykinin-potentiating peptides and related peptides, like the svMP inhibitors, pEKW, pEQW, pEEW and pENW. In preliminary MTT cytotoxicity assays, the highest cancerous-cytotoxicity of crude venom was measured against human neuroblastoma SH-SY5Y cells and shows disintegrin-like effects in some fractions.en_US
dc.description.sponsorshipGerman Research Foundation (DFG)German Research Foundation (DFG); Open Access Publication Funds of Technische Universitat Berlinen_US
dc.description.sponsorshipWe acknowledge support from the German Research Foundation (DFG) and the Open Access Publication Funds of Technische Universitat Berlin.en_US
dc.identifier.doi10.3390/molecules23081893en_US
dc.identifier.issn1420-3049
dc.identifier.issue8en_US
dc.identifier.pmid30060607en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/molecules23081893
dc.identifier.urihttps://hdl.handle.net/11454/30131
dc.identifier.volume23en_US
dc.identifier.wosWOS:000445295500058en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectsnake venomicsen_US
dc.subjectviperidaeen_US
dc.subjectProtobothrops flavoviridisen_US
dc.subjectHabu pit viperen_US
dc.subjectbottom-upen_US
dc.subjecttop-downen_US
dc.subjectBPPen_US
dc.subjecttripeptide metalloprotease inhibitoren_US
dc.subjectcytotoxicityen_US
dc.titleComprehensive Snake Venomics of the Okinawa Habu Pit Viper, Protobothrops flavoviridis, by Complementary Mass Spectrometry-Guided Approachesen_US
dc.typeArticleen_US

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