Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey

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dc.contributor.authorDoskaya, Mert
dc.contributor.authorPullukcu, Husnu
dc.contributor.authorKarakavuk, Muhammet
dc.contributor.authorSahar, Esra Atalay
dc.contributor.authorTasbakan, Mehmet Sezai
dc.contributor.authorTasbakan, Meltem Isikgoz
dc.contributor.authorGuruz, Adnan Yuksel
dc.date.accessioned2020-12-01T12:05:51Z
dc.date.available2020-12-01T12:05:51Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.descriptionkarakavuk, muhammet/0000-0002-2468-5564; Can, Huseyin/0000-0001-9633-9786en_US
dc.description.abstractBackground: Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. in this study, we aimed to detect T. gondii RE gene in various human samples by in house and commercial real time polymerase chain reactions. Methods: A total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An in house and a commercial RT-PCR were applied to investigate the T. gondii RE gene in these samples. Results: the compatibility rate of the two tests was 94.7% (37/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of in house RT-PCR test was 87.5 and 100%. When the in house RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content. Conclusions: Both the commercial and in house RT-PCR tests can be used to investigate T. gondii RE gene in various clinical specimens with their high sensitivity and specificity. in house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, TurkeyEge University [2013-TIP-050]en_US
dc.description.sponsorshipThis study was partly supported by the grant given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant No: 2013-TIP-050) to Y.G. the funding body does not have any role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.en_US
dc.identifier.doi10.1186/s12879-019-4666-zen_US
dc.identifier.issn1471-2334
dc.identifier.issue1en_US
dc.identifier.pmid31823777en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1186/s12879-019-4666-z
dc.identifier.urihttps://hdl.handle.net/11454/63094
dc.identifier.volume19en_US
dc.identifier.wosWOS:000511157700003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofBmc Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectToxoplasma gondiien_US
dc.subjectRE geneen_US
dc.subjectDiagnosisen_US
dc.subjectReal time PCRen_US
dc.subjectin houseen_US
dc.titleComparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkeyen_US
dc.typeArticleen_US

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