Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150

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dc.contributor.authorKarlitepe A.
dc.contributor.authorKabadayi H.
dc.contributor.authorVatansever S.
dc.contributor.authorGurdal M.
dc.contributor.authorGunduz C.
dc.contributor.authorErcan G.
dc.date.accessioned2019-10-26T21:16:14Z
dc.date.available2019-10-26T21:16:14Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-Transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-Transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-Transfected cells, the increased expression of NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy. Copyright © Experimental Oncology, 2017.en_US
dc.description.sponsorshipThis study was supported by grant No. 214S650 from the Turkish Scientific and Technological Research Institute (TUBITAK). --en_US
dc.identifier.endpage218en_US
dc.identifier.issn1812-9269
dc.identifier.issue3en_US
dc.identifier.pmid28967637en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage212en_US
dc.identifier.urihttps://hdl.handle.net/11454/16170
dc.identifier.volume39en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMorion LLCen_US
dc.relation.ispartofExperimental Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipose tissue derived mesenchymal stem cellsen_US
dc.subjectMirna150en_US
dc.subjectNatural killer cellsen_US
dc.subjectPancreatic canceren_US
dc.titleAnti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150en_US
dc.typeArticleen_US

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