Evaluation of significant gene expression changes in congenital and acquired cholesteatoma

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dc.contributor.authorKaya, Isa
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorSahin, Fetih Furkan
dc.contributor.authorOzates, Neslihan Pinar
dc.contributor.authorSezgin, Baha
dc.contributor.authorKurt, Cansu Caliskan
dc.contributor.authorKirazli, Tayfun
dc.date.accessioned2020-12-01T11:58:46Z
dc.date.available2020-12-01T11:58:46Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractEtiopathogenesis of acquired and congenital cholesteatoma is still unclear. the clinical behavior of adult acquired, pediatric acquired and congenital cholesteatomas show differences. the scope of the this study was to detect thematrix metalloproteinase(MMP),tissue inhibitors of metalloproteinase(TIMP) andepidermal growth factor receptor(EGFR) gene expression changes in cholesteatoma perimatrix and to compare these changes among congenital cholesteatoma, adult acquired cholesteatoma and pediatric acquired cholesteatoma. A total of 16 genes includingMMPs,TIMPs andEGFRwere analyzed in the samples of 32 cholesteatoma tissues. Real-time PCR was used for detection of the gene expression levels. Data analyses were achieved by Delta Delta CT method (Light Cycler 480 Quantification Software) and Statistical Package for Social Sciences (SPSS) version 22.0. the expression levels ofMMP-2,-9,-10,-11,-13,-14,-15,-16andEGFRgenes were significantly higher in acquired cholesteatoma than healthy tissue (p < 0.05). There was a statistically significant decrease (3.34 times more) in the meanTIMP-2gene expression level in acquired cholesteatoma compared to healthy tissue (p < 0.05). There was a significant increase in the mean expression level ofMMP-7gene and a decrease in the mean expression level ofTIMP-1gene (3.12 times more) in congenital cholesteatoma compared to healthy tissue (p < 0.05). This study indicates that increased expression levels of some particularMMPgenes andEGFRgene and decreased expression levels ofTIMPgenes may play an important role in the development of cholesteatoma. Further,MMP-9,MMP-13andMMP-14genes may have a remarkable role in the development of more aggressive cholesteatoma forms. the authors concluded that overexpression ofMMP-9,MMP-13andMMP-14may cause stronger inflammation associated with cholesteatoma.en_US
dc.identifier.doi10.1007/s11033-020-05689-9en_US
dc.identifier.endpage6133en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue8en_US
dc.identifier.pmid32740796en_US
dc.identifier.scopus2-s2.0-85088858618en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage6127en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-020-05689-9
dc.identifier.urihttps://hdl.handle.net/11454/62105
dc.identifier.volume47en_US
dc.identifier.wosWOS:000554430700001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCholesteatomaen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectCholesteatoma geneticsen_US
dc.subjectTissue inhibitor of metalloproteinaseen_US
dc.subjectEpidermal growth factor receptoren_US
dc.titleEvaluation of significant gene expression changes in congenital and acquired cholesteatomaen_US
dc.typeArticleen_US

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